library.onepin.app/pregnenolone Peptide Education Library
Education only, not medical advice 21+ Every dose is an example to finalize with a licensed provider

OnePin Peptide Library · Hormonal

Hormonal Peptide Not FDA-approved · investigational

Pregnenolone

Preg

Pregnenolone is an endogenous steroid hormone synthesized from cholesterol, primarily in the adrenal glands, brain, liver, and gonads. It is the precursor to virtually all other steroid hormones, including DHEA, progesterone, cortisol, estrogen, and testosterone. Because of this central role, it is sometimes referred to as the 'mother of all hormones' or the 'master steroid.',Beyond its role as a hormone precursor, pregnenolone is itself a potent neurosteroid. It modulates GABA-A and NMDA receptors in the brain, influencing memory, mood, and cognitive function. Pregnenolone levels decline significantly with age - by age 75, levels may be 60% lower than at age 35 - and this decline has been associated with cognitive changes and mood disturbances.,Pregnenolone is available as an over-the-counter dietary supplement in the United States. It is also prescribed by physicians as part of hormone optimization protocols, particularly in cases of adrenal insufficiency or as a complement to other hormone replacement therapy. While it has a long history of use and a good safety profile at standard doses, large-scale clinical trials are limited.

Quick Start
Route
Oral
Start low
25-100mg
Frequency
daily
Timing
May take with food for better absorption

Start here. Every protocol below is ordered lowest-first. Begin at the smallest effective dose, hold about a week to assess tolerance, and step up only if needed.

Tiered Protocols · lowest first
Quick Start
Conservative start
25-100mg · Oral · daily
Lowest starting point. Hold about a week to assess tolerance before stepping up.
25-100mg
daily
Standard
Neurosteroid Support
50-100 mg · Oral · Daily
human clinical trial · Marx et al. 2009 (Neuropsychopharmacology)
50-100 mg
Daily
Reconstitution CalculatorU-100
050100u
Draw to
units
01

How it works

Serves as the biosynthetic precursor to all major steroid hormones via enzymatic conversion: to progesterone (via 3-beta-HSD), to DHEA (via CYP17A1), and subsequently to downstream androgens, estrogens, and corticosteroids.,Acts as a neurosteroid that positively modulates NMDA receptor activity (enhancing excitatory neurotransmission and memory) while negatively modulating GABA-A receptors (providing mild alerting and mood-elevating effects).,Enhances myelination and neuronal membrane stability in the central nervous system, supporting nerve signal conduction and potentially neuroprotective processes.

Serves as the biosynthetic precursor to all major steroid hormones via enzymatic conversion: to progesterone (via 3-beta-HSD), to DHEA (via CYP17A1), and subsequently to downstream androgens, estrogens, and corticosteroids.,Acts as a neurosteroid that positively modulates NMDA receptor activity (enhancing excitatory neurotransmission and memory) while negatively modulating GABA-A receptors (providing mild alerting and mood-elevating effects).,Enhances myelination and neuronal membrane stability in the central nervous system, supporting nerve signal conduction and potentially neuroprotective processes.

02

What to expect

Early
Days 1–7

Days 1-7: Mild improvements in mood, clarity, and well-being. Some users notice effects within the first few days.

03

Evidence

HumanPresent
AnimalPresent
In-vitroPresent
2009Proof-of-concept trial with the neurosteroid pregnenolone targeting cognitive and negative symptoms in schizophrenia · Marx CE et al, Neuropsychopharmacology ↗peer reviewed
2014Proof-of-concept randomized controlled trial of pregnenolone in schizophrenia · Ritsner MS et al, Psychopharmacology ↗peer reviewed
2014Adjunctive Pregnenolone Ameliorates the Cognitive Deficits in Recent-Onset Schizophrenia: An 8-Week, Randomized, Double-Blind, Placebo-Controlled Trial · Kreinin A et al, Clinical Schizophrenia & Related Psychoses ↗peer reviewed
2014A Randomized, Double-Blind, Placebo-Controlled Trial of Pregnenolone for Bipolar Depression · Brown ES et al, Neuropsychopharmacology ↗peer reviewed
04

Interactions & stacking

What's safe to combine, and what belongs in a separate pin.

Safe & synergistic

MK-677Hormone precursor and GH secretagogue act through independent endocrine pathways
RapamycinNeurosteroid support and mTOR inhibition address different longevity mechanisms
Urolithin AMitochondrial health and hormone precursor support are independent pathways
EpithalonTelomerase activation and steroid precursor supplementation do not conflict

Keep separate

AnastrozolePregnenolone can convert to estrogen via downstream metabolism - aromatase inhibitors may block beneficial conversion while creating hormone imbalance. Monitor levels carefully if combining
05

Pharmacokinetics

Illustrative plasma concentration · 24 h
Half-life
~1-2 hours oral (pregnenolone itself); downstream metabolites have variable half-lives
Peak · Tmax
1-2 hours oral
Elimination
~5-10 hours (parent compound); metabolites persist much longer
Activity
12-24 hours (parent compound); downstream hormone effects persist days

Storage. Store at room temperature in a cool, dry place. Oral compound - no reconstitution needed.

06

Regulatory status

Available over-the-counter as a dietary supplement in the United States. Prescription required in many other countries. Not FDA-approved for any specific medical condition.

Put it to work

Calculate, log & track

Open Pregnenolone straight into OnePin with your vial and dose pre-filled, then let it handle the syringe math and remind you before the vial runs out.

Open Pregnenolone in the app →

Go deeper

The guide & community

The complete Pregnenolone walkthrough, real protocol discussion and coaching live inside the BlessUp community on Skool.

Join the community ↗
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