Hormonal Dietary supplement

Pregnenolone

Preg

Evidence: Studied in humans. Example dose: finalize with a provider. Not a fact-check stamp.

25-100mg – 50-100 mg Oral daily

Pregnenolone is an endogenous steroid hormone synthesized from cholesterol, primarily in the adrenal glands, brain, liver, and gonads. It is the precursor to virtually all other steroid hormones, including DHEA, progesterone, cortisol, estrogen, and testosterone. Because of this central role, it is sometimes referred to as the 'mother of all hormones' or the 'master steroid.'

Quick Start

Route

Oral

Start low

25-100mg Oral

Frequency

daily

Timing

May take with food for better absorption

❋

Start here. Every protocol below is ordered lowest-first. Begin at the smallest effective dose, hold about a week to assess tolerance, and step up only if needed.

Tiered Protocols · lowest first

Conservative start

25-100mg · Oral · daily

conservative starter

25-100mg

daily

Standard

Neurosteroid Support

50-100 mg · Oral · Daily

human clinical trial · Marx et al. 2009 (Neuropsychopharmacology)

Precursor hormone to DHEA, Progesterone, and Cortisol. Highly recommended for patients on TRT or HPA-suppressive therapies.

50-100 mg

Daily

◆

How it works

Serves as the biosynthetic precursor to all major steroid hormones via enzymatic conversion: to progesterone (via 3-beta-HSD), to DHEA (via CYP17A1), and subsequently to downstream androgens, estrogens, and corticosteroids.

Acts as a neurosteroid that positively modulates NMDA receptor activity (enhancing excitatory neurotransmission and memory) while negatively modulating GABA-A receptors (providing mild alerting and mood-elevating effects).

Enhances myelination and neuronal membrane stability in the central nervous system, supporting nerve signal conduction and potentially neuroprotective processes.

What to expect

Early

Days 1–7

Days 1-7: Mild improvements in mood, clarity, and well-being. Some users notice effects within the first few days.

Evidence

HumanPresent

AnimalPresent

In-vitroPresent

Each reference below was re-fetched from its PubMed record, and the quoted sentence was confirmed to appear in that abstract and to support the claim it sits under. Follow any of them to read the source.

Further, pregnenolone administration results in elevations in downstream neurosteroids such as allopregnanolone, a molecule with neuroprotective effects that also increases neurogenesis, decreases apoptosis and inflammation, modulates the hypothalamic-pituitary-adrenal axis, and markedly increases GABA(A) receptor responses.

Further, pregnenolone administration results in elevations in downstream neurosteroids such as allopregnanolone, a molecule with neuroprotective effects that also increases neurogenesis, decreases apoptosis and inflammation, modulates the hypothalamic-pituitary-adrenal axis, and markedly increases GABA(A) receptor responses.

Pregnenolone as a novel therapeutic candidate in schizophrenia: emerging preclinical and clinical evidence. Neuroscience · 2011 · PMID 21756978

Evidence scope. Review of schizophrenia evidence; supports downstream neurosteroid conversion, not the full steroidogenic pathway at the card dose.

Claims we could not support

No abstract we checked supports these for this molecule at this route. That is not the same as saying they are false — it marks where the evidence is missing.

Dose/route: 25-100mg via Oral, daily. No checked live PubMed abstract supported this complete molecule/formulation/route/dose/frequency combination.

Primary safety claim: Mild headache (uncommon). No checked live abstract directly established this as the primary concern for the card's exact formulation and regimen.

Interactions & stacking

Two different questions: what is documented about running these together, and what is documented about drawing them into the same syringe. A good pharmacological partner can still have to be pinned separately.

Documented together

MK-677Hormone precursor and GH secretagogue act through independent endocrine pathways

RapamycinNeurosteroid support and mTOR inhibition address different longevity mechanisms

Urolithin AMitochondrial health and hormone precursor support are independent pathways

EpithalonTelomerase activation and steroid precursor supplementation do not conflict

Keep separate

AnastrozolePregnenolone can convert to estrogen via downstream metabolism - aromatase inhibitors may block beneficial conversion while creating hormone imbalance. Monitor levels carefully if combining

Check any specific pair in the Stack & Interaction Checker, which answers both questions separately. Absence of a documented conflict is not evidence of safety.

Pharmacokinetics

Illustrative plasma concentration · 8 h

Half-life

~1-2 hours

Oral (pregnenolone itself); downstream metabolites have variable half-lives

Peak · Tmax

1-2 hours

Oral

Elimination

~5-10 hours

(parent compound); metabolites persist much longer

Bioavailability

~10-20%

Oral (significant first-pass metabolism)

Duration of action

12-24 hours

(parent compound); downstream hormone effects persist days

Clearance

Hepatic (CYP enzymes); converted to downstream steroid metabolites

Storage. Store at room temperature in a cool, dry place. Oral compound - no reconstitution needed.

Regulatory status

Available over-the-counter as a dietary supplement in the United States. Prescription required in many other countries. Not FDA-approved for any specific medical condition.

Put it to work

Calculate, log & track

Open Pregnenolone straight into OnePin with your vial and dose pre-filled, then let it handle the syringe math and remind you before the vial runs out.

Open Pregnenolone in the app →

Go deeper

The guide & community

The complete Pregnenolone walkthrough, real protocol discussion and coaching live inside the BlessUp community on Skool.

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