OnePin Peptide Library · Longevity
Rapamycin
Sirolimus · Rapamune
Rapamycin (sirolimus) is a macrolide compound originally isolated from the bacterium Streptomyces hygroscopicus found in soil samples from Easter Island (Rapa Nui) in 1972. It is FDA-approved as an immunosuppressant to prevent organ transplant rejection (brand name Rapamune, approved 1999) and is also used in drug-eluting coronary stents. It works by inhibiting the mechanistic target of rapamycin (mTOR), a kinase that serves as a central regulator of cell growth, metabolism, and aging.,Rapamycin has become one of the most studied compounds in longevity research. It is the only drug consistently shown to extend lifespan across multiple species, including yeast, worms, flies, and mice. The National Institute on Aging's Interventions Testing Program confirmed that rapamycin extends mouse lifespan even when started late in life. This has generated enormous interest in low-dose, intermittent rapamycin protocols for human healthspan extension.,At immunosuppressive doses (daily use), rapamycin has well-documented side effects including immunosuppression, hyperlipidemia, and impaired wound healing. However, the longevity community uses intermittent low-dose protocols (typically weekly) which may achieve partial mTOR inhibition with a different, potentially more favorable side effect profile. This distinction between chronic high-dose and intermittent low-dose rapamycin is critical but not yet fully characterized in human clinical trials.
Start here. Every protocol below is ordered lowest-first. Begin at the smallest effective dose, hold about a week to assess tolerance, and step up only if needed.
How it works
Binds to the intracellular protein FKBP12, forming a complex that inhibits mTOR complex 1 (mTORC1), a master regulator of cell growth, protein synthesis, and autophagy. Inhibition of mTORC1 activates autophagy and reduces cellular senescence.,Upregulates autophagy - the cellular recycling process that clears damaged proteins and organelles - which declines with age and is thought to be a key mechanism behind its lifespan extension effects.,At immunosuppressive doses, also inhibits mTOR complex 2 (mTORC2) with chronic exposure, which affects insulin signaling and lipid metabolism. Intermittent dosing preferentially targets mTORC1 while sparing mTORC2.
Binds to the intracellular protein FKBP12, forming a complex that inhibits mTOR complex 1 (mTORC1), a master regulator of cell growth, protein synthesis, and autophagy. Inhibition of mTORC1 activates autophagy and reduces cellular senescence.,Upregulates autophagy - the cellular recycling process that clears damaged proteins and organelles - which declines with age and is thought to be a key mechanism behind its lifespan extension effects.,At immunosuppressive doses, also inhibits mTOR complex 2 (mTORC2) with chronic exposure, which affects insulin signaling and lipid metabolism. Intermittent dosing preferentially targets mTORC1 while sparing mTORC2.
What to expect
Weeks 1-4: Minimal subjective changes. Drug is building tissue levels. Mouth sores possible (usually mild at low doses).
Evidence
Interactions & stacking
What's safe to combine, and what belongs in a separate pin.
Safe & synergistic
Keep separate
Pharmacokinetics
Storage. Store at room temperature (20-25C). Protect from light. Oral compound - no reconstitution needed.
Regulatory status
FDA-approved (Rapamune/sirolimus) for renal transplant rejection prevention and lymphangioleiomyomatosis. Use for longevity/aging is off-label. Prescription required.
Put it to work
Calculate, log & track
Open Rapamycin straight into OnePin with your vial and dose pre-filled, then let it handle the syringe math and remind you before the vial runs out.
Open Rapamycin in the app →Go deeper
The guide & community
The complete Rapamycin walkthrough, real protocol discussion and coaching live inside the BlessUp community on Skool.
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