library.onepin.app/epithalon Peptide Education Library
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OnePin Peptide Library · Longevity

Longevity Synthetic tetrapeptide Not FDA-approved · investigational Controlled substance

Epithalon

Epitalon · AEDG · Epithalamin

Synthetic tetrapeptideKhavinson bioregulatorTelomerase activatorPineal gland regulator

Epithalon (also spelled Epitalon) is a synthetic tetrapeptide (Ala-Glu-Asp-Gly) developed by Professor Vladimir Khavinson at the St. Petersburg Institute of Bioregulation and Gerontology. It is a Khavinson bioregulator, a class of short peptides that regulate gene expression in specific tissues at very low concentrations. Epithalon targets the pineal gland and activates telomerase, the enzyme responsible for maintaining telomere length on chromosomal ends. Telomere shortening is one of the primary hallmarks of cellular aging. Epithalon is the synthetic analog of Epithalamin, a peptide extract originally derived from bovine pineal gland. It is administered in short, intensive courses (10-20 days) rather than continuously, consistent with Khavinson's bioregulatory dosing philosophy.

Quick Start
Route
SubQ injection
Start low
5-10 mg daily
Frequency
Daily for 10-20 day course, then extended rest
Timing
No evidence-based fed or fasted requirement. Evening dosing sometimes preferred for pineal/melatonin alignment.

Evening/bedtime dosing preferred (pineal gland targeting, melatonin relationship). 10-20 day courses. Pin alone.

Start here. Every protocol below is ordered lowest-first. Begin at the smallest effective dose, hold about a week to assess tolerance, and step up only if needed.

Tiered Protocols · lowest first
Quick Start
Conservative start
5-10 mg daily · SubQ injection · Daily for 10-20 day course, then extended rest
Lowest starting point. Hold about a week to assess tolerance before stepping up.
5-10 mg daily
Daily for 10-20 day course, then extended rest
Intermediate
Longevity Reset Protocol
10 mg · SubQ · Daily
in vitro · Khavinson, Bondarev & Butyugov 2003 (Bulletin of Experimental Biology and Medicine, 135(6):590-592)
10 mg
Daily
Reconstitution CalculatorU-100
050100u
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units
01

How it works

Epithalon activates the catalytic subunit of telomerase (hTERT) in somatic cells, enabling the enzyme to add telomeric repeats (TTAGGG) to chromosomal ends. This counteracts the progressive telomere shortening that occurs with each cell division and is one of the recognized hallmarks of aging. Epithalon also normalizes pineal gland function, restoring melatonin production patterns that decline with age. Melatonin itself has antioxidant and immunomodulatory properties. As a Khavinson bioregulator, Epithalon works through gene expression regulation at very low concentrations, interacting with DNA at the level of histone modification and transcription factor accessibility rather than through traditional receptor binding. This mechanism explains why bioregulators are administered in short intensive courses followed by extended rest periods.

Epithalon activates the catalytic subunit of telomerase (hTERT) in somatic cells, enabling the enzyme to add telomeric repeats (TTAGGG) to chromosomal ends. This counteracts the progressive telomere shortening that occurs with each cell division and is one of the recognized hallmarks of aging. Epithalon also normalizes pineal gland function, restoring melatonin production patterns that decline with age. Melatonin itself has antioxidant and immunomodulatory properties. As a Khavinson bioregulator, Epithalon works through gene expression regulation at very low concentrations, interacting with DNA at the level of histone modification and transcription factor accessibility rather than through traditional receptor binding. This mechanism explains why bioregulators are administered in short intensive courses followed by extended rest periods.

02

What to expect

Early
Days 1–7

Days 1-10 (during course): Sleep quality improvements may be noticeable (pineal/melatonin normalization). Gene expression changes are initiated but not yet manifested clinically. Course is only 10-20 days.

Mid
Weeks 2–4

Weeks 2-12 (after course): Gene expression changes continue to manifest. Telomerase activation effects persist beyond the dosing period. Sleep improvements may sustain.

Later
Weeks 4–12

Months 3-12: Cumulative effects across multiple courses. Telomere length measurements (if tracked) may show stabilization or modest lengthening over time. Best assessed through longitudinal biomarker tracking.

03

Evidence

HumanModerate
AnimalModerate
In-vitroPresent
2003Epithalon peptide induces telomerase activity and telomere elongation in human somatic cells · Khavinson VKh, Bondarev IE, Butyugov AA, Bulletin of Experimental Biology and Medicine ↗peer reviewed
2025Epitalon increases telomere length in human cell lines through telomerase upregulation or ALT activity · Pinheiro D, Tibone M, Carvalho L, et al, Aging ↗peer reviewed
2010Peptide bioregulation of aging: results and prospects · Anisimov VN, Khavinson VKh, Biogerontology ↗review
2009Peptide regulation of aging: 35-year research experience · Khavinson VKh, Anisimov VN, Bulletin of Experimental Biology and Medicine ↗review
04

The honest take

Telomerase activation

Supported

Confirmed in multiple cell culture studies showing hTERT activation and telomere elongation. The mechanism is well-supported. Whether this translates to meaningful anti-aging effects in humans requires long-term data.

Lifespan extension
Supported

Animal studies show approximately 25% lifespan increase in rodents. One of the few compounds with lifespan data. Not confirmed in humans.

Pineal / melatonin restoration

Animal data shows normalization of melatonin production in aged animals. Consistent with Khavinson's bioregulatory theory of organ-specific gene expression restoration.

05

Interactions & stacking

What's safe to combine, and what belongs in a separate pin.

Safe & synergistic

SS-31Epithalon targets telomere maintenance (cellular lifespan), SS-31 targets mitochondrial membrane integrity (cellular energy). Two fundamental hallmarks of aging addressed simultaneously. Pin separately.
PinealonBoth target pineal gland through different bioregulatory mechanisms. Can be run in sequence for extended pineal support.

Keep separate

GHK-CuCopper ions from GHK-Cu degrade Epithalon's peptide structure. Never mix in the same syringe. Separate injection sites recommended when dosed close in time.
All other peptidesPIN ALONE. Khavinson bioregulators work through gene expression regulation at extremely low effective concentrations. Mixing with other peptides could interfere with the precise signaling mechanism.
06

Side effects & safety

Commonly reported

  • Very well tolerated
  • Mild injection site irritation
  • No significant side effects reported in published literature

Less common & notes

  • Epithalon is a small tetrapeptide with a clean safety profile in published research.
  • The short course duration (10-20 days) limits cumulative exposure.
  • Theoretical concern about telomerase activation in cancer cells, though animal studies actually showed reduced tumor incidence.
  • Individuals with active malignancy should consult their oncologist before use.
07

Pharmacokinetics

Illustrative plasma concentration · 24 h
Half-life
0.5h
Peak · Tmax
0.25h
Elimination
2.5h
Activity
Months (telomerase activation and pineal gene expression changes persist for months after a 10-20 day course)

Storage. Store unreconstituted vials frozen (-20C) or refrigerated (2-8C). After reconstitution with BAC water, refrigerate at 2-8C. Stable for 28-30 days. Small tetrapeptide with favorable stability. Short 10-day course means reconstituted vial is typically used within 5-10 days, well within stability window.

08

Regulatory status

Epithalon is not FDA-approved for any indication. Available as a research peptide. Used in Russian anti-aging medicine. WADA status: Not specifically listed. Not a controlled substance.

Put it to work

Calculate, log & track

Open Epithalon straight into OnePin with your vial and dose pre-filled, then let it handle the syringe math and remind you before the vial runs out.

Open Epithalon in the app →

Go deeper

The guide & community

The complete Epithalon walkthrough, real protocol discussion and coaching live inside the BlessUp community on Skool.

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