library.onepin.app/ketamine Peptide Education Library
Education only, not medical advice 21+ Every dose is an example to finalize with a licensed provider
Cognitive & Mood FDA-approved medicineControlled substance

Ketamine

Ketamine HCl · Ketalar

0.5mg/kg IV over 40 min (clinical standard for depression) – 0.5 mg/kg IM 2-3x per week for initial series (6 infusions), then monthly maintenance

Ketamine is a dissociative anesthetic first synthesized in 1962 and FDA-approved since 1970 for anesthesia and analgesia. It acts primarily as an NMDA receptor antagonist and has emerged as a breakthrough treatment for depression, particularly treatment-resistant depression (TRD). The S-enantiomer (esketamine, marketed as Spravato) received FDA approval in 2019 for TRD in conjunction with an oral antidepressant.

Quick Start
Route
IV, IM, Sublingual, or Intranasal
Start low
0.5mg/kg IM
Frequency
2-3x per week for initial series (6 infusions), then monthly maintenance
Timing
Anytime

Starting dose. 0.5mg/kg — IV over 40 min (clinical standard for depression)

Clinical setting with monitoring. No driving for 24 hours post-treatment. Empty stomach preferred to reduce nausea.

Start here. Every protocol below is ordered lowest-first. Begin at the smallest effective dose, hold about a week to assess tolerance, and step up only if needed.

Tiered Protocols · lowest first
Conservative start
0.5mg/kg IV over 40 min (clinical standard for depression) · IV, IM, Sublingual, or Intranasal · 2-3x per week for initial series (6 infusions), then monthly maintenance
conservative starter
0.5mg/kg IV over 40 min (clinical standard for depression)
2-3x per week for initial series (6 infusions), then monthly maintenance
Expert
Depression / Neuroplasticity Reset
0.5 mg/kg · IM · 2x Weekly
human clinical trial · Berman et al. 2000 (Biol Psychiatry)
Route listed as IM. Clinical standard is slow IV infusion over 40 minutes at 0.5mg/kg. Requires clinical monitoring. Note the route: Berman 2000 used a single INTRAVENOUS infusion. This card is intramuscular. IM absorption is high but not identical, and the trial was a supervised clinical setting.
0.5 mg/kg
2x Weekly
01

How it works

Ketamine is a non-competitive NMDA (N-methyl-D-aspartate) receptor antagonist that blocks the ion channel at the PCP binding site. At sub-anesthetic doses used for depression, it preferentially blocks NMDA receptors on GABAergic interneurons, disinhibiting glutamatergic pyramidal neurons. This produces a transient surge of glutamate in the prefrontal cortex and hippocampus.

The glutamate surge activates AMPA receptors, which triggers downstream signaling through BDNF (brain-derived neurotrophic factor) and the mTOR pathway. This cascade promotes rapid dendritic spine growth and synaptogenesis - literally creating new synaptic connections - within hours. This synaptic plasticity mechanism explains both the rapid onset and the transient nature of ketamine's antidepressant effect.

Additional mechanisms include opioid receptor modulation (mu and kappa), HCN1 channel blockade, and anti-inflammatory effects via inhibition of microglial activation and reduction of pro-inflammatory cytokines. The S-enantiomer (esketamine) has approximately 4x higher NMDA affinity than racemic ketamine.

02

What to expect

Early
Days 1–7

Hours 2-24: Rapid antidepressant onset. Dissociative effects resolve within 2 hours. Mood improvement often noted same day.

03

Evidence

HumanPresent
AnimalPresent
In-vitroPresent

References for this compound are being re-checked against their source records. We are not showing them until each one is confirmed to support the claim it sits under.

04

Interactions & stacking

Two different questions: what is documented about running these together, and what is documented about drawing them into the same syringe. A good pharmacological partner can still have to be pinned separately.

Documented together

NAD+NAD+ supports mitochondrial function and cellular energy independent of ketamine's glutamatergic mechanism
Lion's ManeNGF promotion from lion's mane may complement ketamine-induced synaptogenesis through parallel neurotrophic support
MagnesiumMagnesium is a natural NMDA receptor modulator - may enhance ketamine's neuroplastic effects and reduce dissociative side effects
PsilocybinBoth promote neuroplasticity through different mechanisms (NMDA vs 5-HT2A). Some clinicians explore sequential or combined protocols for treatment-resistant depression

Keep separate

BenzodiazepinesBenzodiazepines enhance GABAergic inhibition, which opposes ketamine's mechanism of disinhibiting glutamate release. They reduce antidepressant efficacy while increasing sedation risk
Lamotrigine (at time of dosing)Lamotrigine blocks glutamate release, which may attenuate ketamine's acute antidepressant mechanism. Some clinicians hold lamotrigine on infusion days
CNS Depressants (opioids, alcohol)Additive CNS and respiratory depression risk. Opioid antagonists (naltrexone) may also block some of ketamine's antidepressant effects
MAOIsRisk of hypertensive crisis and unpredictable sympathomimetic effects when combined with ketamine

Check any specific pair in the Stack & Interaction Checker, which answers both questions separately. Absence of a documented conflict is not evidence of safety.

05

Pharmacokinetics

Illustrative plasma concentration · 10 h
Half-life
~2-3 hours

(ketamine), ~5 hours (norketamine active metabolite)

Peak · Tmax
~1 min

IV, ~5-15 min IM, ~20-30 min sublingual, ~20-40 min intranasal

Elimination
~12-17 hours

Including active metabolites

Bioavailability
~100%

IV, ~93% IM, ~25-30% sublingual, ~45-50% intranasal, ~17-20% oral

Duration of action

Dissociative effects: 45-90 min IV, 1-2 hr IM, 1-3 hr sublingual. Antidepressant effects: 1-3 weeks per treatment.

Clearance

Hepatic (CYP3A4 and CYP2B6 N-demethylation to norketamine). Renal excretion of hydroxylated metabolites and conjugates.

Storage. Store vials at controlled room temperature (20-25C). Protect from light. Sublingual troches should be refrigerated for extended storage. Esketamine (Spravato) nasal spray devices stored at room temperature in original packaging.

06

Regulatory status

Schedule III controlled substance (DEA). FDA-approved for anesthesia (1970) and esketamine (Spravato) approved for treatment-resistant depression (2019) under REMS program. Racemic ketamine used off-label for depression via IV, IM, sublingual, and intranasal routes. Spravato must be administered in certified healthcare settings with post-dose monitoring.

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