library.onepin.app/lion-s-mane Peptide Education Library
Education only, not medical advice 21+ Every dose is an example to finalize with a licensed provider

OnePin Peptide Library · Cognitive & Mood

Cognitive & Mood Peptide Not FDA-approved · investigational

Lion's Mane

Hericium erinaceus · Yamabushitake

Lion's mane (Hericium erinaceus) is an edible medicinal mushroom with a long history of use in Traditional Chinese Medicine. It is unique among medicinal mushrooms for its well-documented neurotrophic properties - specifically the ability to stimulate nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) synthesis through its bioactive compounds hericenones (found in the fruiting body) and erinacines (found in the mycelium).,Lion's mane has become one of the most popular nootropic supplements in the biohacking community, used for cognitive enhancement, neuroprotection, and nerve regeneration. It is also a key component of the Stamets Stack (lion's mane + psilocybin microdose + niacin) for neuroplasticity. Beyond cognitive effects, lion's mane demonstrates anti-inflammatory, immunomodulatory, and gastroprotective properties.,The evidence base includes several small human RCTs showing cognitive improvement in mild cognitive impairment, along with robust preclinical data for nerve regeneration, myelination, and neuroprotection. Both fruiting body and mycelium extracts are used, though they contain different bioactive profiles - dual extracts or products specifying erinacine/hericenone content are preferred.

Quick Start
Route
Oral
Start low
500-1000mg extract (or 3g whole mushroom powder)
Frequency
1-2x daily
Timing
Anytime

Can be taken with or without food. Morning or split morning/afternoon dosing. Look for products specifying beta-glucan content (>25%) or standardized hericenone/erinacine content.

Start here. Every protocol below is ordered lowest-first. Begin at the smallest effective dose, hold about a week to assess tolerance, and step up only if needed.

Tiered Protocols · lowest first
Quick Start
Conservative start
500-1000mg extract (or 3g whole mushroom powder) · Oral · 1-2x daily
Lowest starting point. Hold about a week to assess tolerance before stepping up.
500-1000mg extract (or 3g whole mushroom powder)
1-2x daily
Standard
NGF Stimulation
500-1000 mg · Oral · Daily
human clinical trial · Mori et al. 2009 (Phytother Res)
500-1000 mg
Daily
01

How it works

Lion's mane contains two families of bioactive compounds that cross the blood-brain barrier and stimulate neurotrophic factor synthesis. Hericenones (H3, H4, H8, found in fruiting body) and erinacines (A, B, C, found in mycelium) both stimulate NGF synthesis in astrocytes through activation of the JNK and p38 MAPK signaling pathways. NGF is essential for neuronal survival, growth, differentiation, and myelination.,Beyond NGF, lion's mane promotes BDNF expression and enhances myelination through upregulation of myelin basic protein and peripheral myelin protein 22. This dual neurotrophic effect supports both neurogenesis (new neuron formation, particularly in the hippocampus) and remyelination of damaged nerve fibers.,Anti-inflammatory effects are mediated through inhibition of NF-kB signaling and reduction of pro-inflammatory cytokines (TNF-alpha, IL-6). Gastroprotective effects involve protection against H. pylori and promotion of gastric mucosal integrity. Immunomodulatory effects include enhanced gut-associated immune function through polysaccharide-mediated macrophage activation.

Lion's mane contains two families of bioactive compounds that cross the blood-brain barrier and stimulate neurotrophic factor synthesis. Hericenones (H3, H4, H8, found in fruiting body) and erinacines (A, B, C, found in mycelium) both stimulate NGF synthesis in astrocytes through activation of the JNK and p38 MAPK signaling pathways. NGF is essential for neuronal survival, growth, differentiation, and myelination.,Beyond NGF, lion's mane promotes BDNF expression and enhances myelination through upregulation of myelin basic protein and peripheral myelin protein 22. This dual neurotrophic effect supports both neurogenesis (new neuron formation, particularly in the hippocampus) and remyelination of damaged nerve fibers.,Anti-inflammatory effects are mediated through inhibition of NF-kB signaling and reduction of pro-inflammatory cytokines (TNF-alpha, IL-6). Gastroprotective effects involve protection against H. pylori and promotion of gastric mucosal integrity. Immunomodulatory effects include enhanced gut-associated immune function through polysaccharide-mediated macrophage activation.

02

What to expect

Early
Days 1–7

Weeks 1-2: Minimal observable effects. NGF upregulation beginning at cellular level.

03

Evidence

HumanPresent
AnimalStrong
In-vitroPresent
2009Improving effects of the mushroom Yamabushitake (Hericium erinaceus) on mild cognitive impairment: a double-blind placebo-controlled clinical trial · Mori K et al, Phytotherapy Research ↗peer reviewed
2019Improvement of cognitive functions by oral intake of Hericium erinaceus · Saitsu Y et al, Biomedical Research ↗peer reviewed
2023The Acute and Chronic Effects of Lion's Mane Mushroom Supplementation on Cognitive Function, Stress and Mood in Young Adults: A Double-Blind, Parallel Groups, Pilot Study · Docherty S et al, Nutrients ↗peer reviewed
2025Acute effects of a standardised extract of Hericium erinaceus (Lion's Mane mushroom) on cognition and mood in healthy younger adults: a double-blind randomised placebo-controlled study · Roeder D et al, Frontiers in Nutrition ↗peer reviewed
04

Interactions & stacking

What's safe to combine, and what belongs in a separate pin.

Safe & synergistic

ModafinilComplementary cognitive mechanisms - modafinil for acute alertness, lion's mane for long-term neurotrophic support
Magnesium ThreonateBoth support cognitive function - magnesium via NMDA modulation, lion's mane via NGF stimulation
Psilocybin (microdose)The Stamets Stack - lion's mane NGF/BDNF stimulation combines with psilocybin's 5-HT2A neuroplasticity for enhanced neurogenesis
Niacin (flush form)Niacin vasodilation may enhance CNS delivery of lion's mane bioactives (Stamets Stack component)

Keep separate

Anticoagulants (warfarin, heparin)Lion's mane has mild anti-platelet activity - may potentiate bleeding risk with anticoagulant medications
Diabetes Medications (insulin, metformin)Lion's mane may lower blood glucose - additive hypoglycemia risk when combined with diabetes medications. Monitor blood sugar closely
05

Pharmacokinetics

Illustrative plasma concentration · 24 h
Half-life
Not precisely characterized for individual bioactives in humans
Peak · Tmax
~1-2 hours (estimated for hericenones based on limited PK data)
Elimination
Not well-characterized in humans
Activity
NGF stimulation effects persist for the duration of supplementation. Cognitive benefits require weeks of consistent dosing and reverse upon discontinuation.

Storage. Store in a cool, dry place away from light and moisture. Capsules and powders are shelf-stable at room temperature for 1-2 years. Refrigeration extends potency. Liquid extracts (tinctures) should be stored per manufacturer instructions.

06

Regulatory status

Classified as a dietary supplement in the US (not FDA-approved for any medical condition). GRAS (Generally Recognized as Safe) status as a food ingredient. No prescription required. Widely available over the counter. Quality varies significantly between products - look for third-party testing and standardized extracts.

Put it to work

Calculate, log & track

Open Lion's Mane straight into OnePin with your vial and dose pre-filled, then let it handle the syringe math and remind you before the vial runs out.

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Go deeper

The guide & community

The complete Lion's Mane walkthrough, real protocol discussion and coaching live inside the BlessUp community on Skool.

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