library.onepin.app/vip Peptide Education Library
Education only, not medical advice 21+ Every dose is an example to finalize with a licensed provider

OnePin Peptide Library · Immune

Immune Endogenous neuropeptide Not FDA-approved · investigational

VIP

Vasoactive Intestinal Peptide · Aviptadil

Endogenous neuropeptideVPAC1/VPAC2 receptor agonistVasodilator and bronchodilatorImmunomodulatory peptide

VIP (Vasoactive Intestinal Peptide) is a 28-amino acid neuropeptide that functions as a potent vasodilator, bronchodilator, and immunomodulator. It is endogenously produced in the gut, pancreas, brain, and immune cells, acting through VPAC1 and VPAC2 receptors to regulate vascular tone, smooth muscle relaxation, immune cell differentiation, and circadian rhythm maintenance. VIP has been studied in the context of pulmonary arterial hypertension, chronic inflammatory respiratory illness, mast cell activation, neuroinflammation, and GI motility disorders. In peptide protocols, VIP is valued for its anti-inflammatory, bronchodilatory, and neuroprotective properties and is commonly administered as part of the evening GH axis syringe with Tesamorelin and Ipamorelin.

Quick Start
Route
SubQ injection. Also available as nasal spray and inhaled formulations.
Start low
50 mcg, titrating to 100 mcg
Frequency
Once daily
Timing
No strict fasting requirement for VIP itself. If pinned with Tesamorelin/Ipamorelin, fasted for those compounds.

Intranasal: Multiple times daily per CIRS protocol. SubQ: Evening dosing. Very short half-life (~1-2 min IV), nasal route provides more sustained absorption. Use within 2-3 weeks of reconstitution.

Start here. Every protocol below is ordered lowest-first. Begin at the smallest effective dose, hold about a week to assess tolerance, and step up only if needed.

Tiered Protocols · lowest first
Quick Start
Conservative start
50 mcg, titrating to 100 mcg · SubQ injection. Also available as nasal spray and inhaled formulations. · Once daily
Lowest starting point. Hold about a week to assess tolerance before stepping up.
50 mcg, titrating to 100 mcg
Once daily
Expert
CIRS / Mold Toxicity Protocol
50 mcg · Nasal · Daily
human study · Shoemaker, House & Ryan 2013, Health 3:396-401
50 mcg
Daily
Reconstitution CalculatorU-100
050100u
Draw to
units
01

How it works

VIP binds to VPAC1 and VPAC2 receptors, activating adenylate cyclase and increasing intracellular cAMP. This triggers smooth muscle relaxation (vasodilation, bronchodilation), inhibition of pro-inflammatory cytokine release from macrophages and T-cells, mast cell stabilization, and modulation of Th1/Th2 immune balance toward anti-inflammatory Th2 response. VIP also acts as a neurotransmitter and neuromodulator in the central and enteric nervous systems, regulating circadian clock function in the suprachiasmatic nucleus, GI motility, and pancreatic secretion. In immune cells, VIP shifts macrophage polarization from pro-inflammatory M1 to anti-inflammatory M2 phenotype. The methionine at position 17 is critical for receptor binding but is oxidation-sensitive, which creates specific handling and mixing requirements.

VIP binds to VPAC1 and VPAC2 receptors, activating adenylate cyclase and increasing intracellular cAMP. This triggers smooth muscle relaxation (vasodilation, bronchodilation), inhibition of pro-inflammatory cytokine release from macrophages and T-cells, mast cell stabilization, and modulation of Th1/Th2 immune balance toward anti-inflammatory Th2 response. VIP also acts as a neurotransmitter and neuromodulator in the central and enteric nervous systems, regulating circadian clock function in the suprachiasmatic nucleus, GI motility, and pancreatic secretion. In immune cells, VIP shifts macrophage polarization from pro-inflammatory M1 to anti-inflammatory M2 phenotype. The methionine at position 17 is critical for receptor binding but is oxidation-sensitive, which creates specific handling and mixing requirements.

02

What to expect

Early
Days 1–7

Days 1-7: Vasodilatory effects (warmth, mild flushing) may be noticed immediately, particularly at higher doses. Respiratory improvements may begin. GI motility effects within first week.

Mid
Weeks 2–4

Weeks 2-4: Anti-inflammatory effects become more consistent. Respiratory function improvements stabilize. Sleep quality may improve due to circadian rhythm support.

Later
Weeks 4–12

Weeks 4-12: Cumulative immunomodulatory effects. Chronic inflammatory markers may improve. Best assessed through symptom tracking and inflammatory marker labwork (CRP, ESR, cytokine panels).

03

Evidence

HumanStrong
AnimalStrong
In-vitroStrong
2010Inhaled vasoactive intestinal peptide exerts immunoregulatory effects in sarcoidosis · Prasse A, Zissel G, Lutzen N, et al, American Journal of Respiratory and Critical Care Medicine ↗peer reviewed
2003Vasoactive intestinal peptide as a new drug for treatment of primary pulmonary hypertension · Petkov V, Mosgoeller W, Ziesche R, et al, Journal of Clinical Investigation ↗peer reviewed
2008Inhalation of vasoactive intestinal peptide in pulmonary hypertension · Leuchte HH, Baezner C, Baumgartner RA, et al, European Respiratory Journal ↗peer reviewed
2013Vasoactive intestinal peptide inhaled agonists: potential role in respiratory therapeutics · Mathioudakis AG, Chatzimavridou-Grigoriadou V, Evangelopoulou E, Mathioudakis GA, Hippokratia ↗review
04

The honest take

CIRS treatment

Dr. Shoemaker's protocol uses VIP nasal spray as a final step in CIRS treatment. Published case series show improvements in inflammatory markers. Not FDA-approved for CIRS. The most established clinical application for VIP.

Pulmonary hypertension
Supported

Human clinical trial data with inhaled VIP showing improved pulmonary hemodynamics. Mechanism (pulmonary vasodilation) is well-supported. Limited sample sizes.

Mast cell stabilization

Preclinical data supports VIP-mediated mast cell stabilization and reduced histamine release. Relevant for mast cell activation conditions. Not a validated mast cell treatment.

05

Interactions & stacking

What's safe to combine, and what belongs in a separate pin.

Safe & synergistic

TesamorelinVIP adds anti-inflammatory and bronchodilatory support to GH axis syringe. No chemical conflict.
IpamorelinNo chemical conflict. Standard P1 syringe partner.
BPC-157Complementary anti-inflammatory pathways
TB-500Complementary repair pathways

Keep separate

GHK-CuCRITICAL: Copper ions from GHK-Cu catalyze oxidation of VIP's methionine-17 residue, which is essential for receptor binding. This destroys VIP's biological activity. NEVER mix in syringe or inject at the same site.
CJC-1295 with DACDAC maleimide reactivity. Pin CJC-DAC separately. VIP + Ipa can share syringe during CJC-DAC phase.
06

Side effects & safety

Commonly reported

  • Nasal congestion (vasodilation of nasal mucosa)
  • Mild flushing or warmth
  • Transient blood pressure decrease (vasodilation)
  • Loose stools or increased GI motility

Less common & notes

  • VIP is a potent vasodilator and can cause hypotension, particularly in volume-depleted individuals or those on blood pressure medications.
  • Diarrhea at higher doses due to GI smooth muscle effects.
  • Rarely, headache from vasodilation.
  • Start at low dose and titrate up.
  • Contraindicated in individuals with significant hypotension.
  • Monitor blood pressure during titration.
07

Pharmacokinetics

Illustrative plasma concentration · 24 h
Half-life
0.5h
Peak · Tmax
0.17h
Elimination
2.5h
Activity
6-12 hours (immunomodulatory and bronchodilatory effects persist beyond plasma clearance)

Storage. CRITICAL: Methionine-17 is oxidation-sensitive. Protect from light at all times. Reconstitute with BAC water (pH 5-7 acceptable). Refrigerate at 2-8C. Stable for 14-21 days after reconstitution in SubQ solution. Reconstitute fresh every 2-3 weeks regardless of remaining volume. Do not expose to direct light during storage or injection preparation. Unreconstituted lyophilized powder is more stable (store frozen -20C).

08

Regulatory status

VIP is available through compounding pharmacies by prescription. Not FDA-approved for any injectable indication. Available as a research peptide. Not specifically listed on WADA prohibited list. Used clinically in some countries for specific indications.

Put it to work

Calculate, log & track

Open VIP straight into OnePin with your vial and dose pre-filled, then let it handle the syringe math and remind you before the vial runs out.

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The guide & community

The complete VIP walkthrough, real protocol discussion and coaching live inside the BlessUp community on Skool.

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