library.onepin.app/ghk Peptide Education Library
Education only, not medical advice 21+ Every dose is an example to finalize with a licensed provider

OnePin Peptide Library · Repair & Recovery

Repair & Recovery Endogenous tripeptide Not FDA-approved · investigational

GHK

Glycyl-L-Histidyl-L-Lysine · GHK Tripeptide

Endogenous tripeptideGene expression modulatorCopper-free tissue signaling peptide

GHK (glycyl-L-histidyl-L-lysine) is the same tripeptide sequence as GHK-Cu but WITHOUT the copper ion. It retains cell signaling and gene expression modulation properties but lacks the copper-dependent collagen cross-linking and metalloenzyme activation that makes GHK-Cu uniquely potent. Without copper, GHK has no oxidation concerns and is compatible with a wider range of peptides including VIP and bioregulators. GHK is less commonly used than GHK-Cu because the copper complex provides additional biological activity, but it serves as a safer mixing option where copper incompatibility is a concern.

Quick Start
Route
SubQ injection
Start low
1-2 mg daily
Frequency
Daily
Timing
No fasting required

Same timing as GHK-Cu. Evening preferred. No fasting required.

Start here. Every protocol below is ordered lowest-first. Begin at the smallest effective dose, hold about a week to assess tolerance, and step up only if needed.

Tiered Protocols · lowest first
Quick Start
Conservative start
1-2 mg daily · SubQ injection · Daily
Lowest starting point. Hold about a week to assess tolerance before stepping up.
1-2 mg daily
Daily
Standard
Systemic Copper-Free Repair
1.5-2 mg · SubQ · Daily
human study · Pickart et al. 2015 (Biomed Res Int)
1.5-2 mg
Daily
Reconstitution CalculatorU-100
050100u
Draw to
units
01

How it works

GHK modulates gene expression through direct DNA interaction and transcription factor modulation, upregulating genes involved in tissue repair and downregulating inflammatory genes. Without copper, it cannot activate lysyl oxidase or other copper-dependent enzymes, so collagen cross-linking effects are absent. It retains the ability to influence approximately 4,000 human genes in expression studies, though the magnitude of effect is generally less than GHK-Cu.

GHK modulates gene expression through direct DNA interaction and transcription factor modulation, upregulating genes involved in tissue repair and downregulating inflammatory genes. Without copper, it cannot activate lysyl oxidase or other copper-dependent enzymes, so collagen cross-linking effects are absent. It retains the ability to influence approximately 4,000 human genes in expression studies, though the magnitude of effect is generally less than GHK-Cu.

02

What to expect

Early
Days 1–7

Days 1-14: Minimal changes.

Mid
Weeks 2–4

Weeks 2-6: Subtle tissue support.

Later
Weeks 4–12

Weeks 6-12: Cumulative gene expression effects.

03

Evidence

HumanModerate
AnimalModerate
In-vitroPresent
2022The potential of GHK as an anti-aging peptide · Pickart L, Margolina A, Aging Pathobiology and Therapeutics ↗review
2015GHK Peptide as a Natural Modulator of Multiple Cellular Pathways in Skin Regeneration · Pickart L, Vasquez-Soltero JM, Margolina A, BioMed Research International ↗review
2015Anti-Aging Activity of the GHK Peptide - The skin and beyond · Pickart L, Vasquez-Soltero JM, Margolina A, Journal of Aging Research and Clinical Practice ↗review
04

The honest take

Copper-free alternative

Confirmed. GHK without copper is compatible with VIP, bioregulators, and other copper-sensitive peptides. Trade-off is reduced biological potency.

05

Interactions & stacking

What's safe to combine, and what belongs in a separate pin.

Safe & synergistic

VIPNo copper = no methionine-17 oxidation risk. Safe to mix unlike GHK-Cu.
BPC-157Non-overlapping mechanisms
TB-500Non-overlapping mechanisms
IpamorelinNon-overlapping mechanisms

Keep separate

CJC-1295 with DACDAC maleimide reactivity.
06

Side effects & safety

Commonly reported

  • Well tolerated
  • Mild injection site irritation

Less common & notes

  • No copper-related concerns.
  • Excellent safety profile.
07

Pharmacokinetics

Illustrative plasma concentration · 24 h
Half-life
0.5h
Peak · Tmax
0.05h
Elimination
2.5h
Activity
6-12 hours (gene expression changes persist beyond peptide clearance)

Storage. Standard BAC water 2-8C. Stable for 28 days. No copper oxidation concerns.

08

Regulatory status

Available as research peptide. Not FDA-approved. No WADA prohibition.

Put it to work

Calculate, log & track

Open GHK straight into OnePin with your vial and dose pre-filled, then let it handle the syringe math and remind you before the vial runs out.

Open GHK in the app →

Go deeper

The guide & community

The complete GHK walkthrough, real protocol discussion and coaching live inside the BlessUp community on Skool.

Join the community ↗
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