library.onepin.app/i3c Peptide Education Library

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OnePin Peptide Library · Hormonal

Hormonal Indole-3-Carbinol (Cruciferous Phytochemical)

I3C

Indole-3-Carbinol

Indole-3-Carbinol (Cruciferous Phytochemical)Estrogen Metabolism ModulatorPolymerizes to DIM in StomachDietary Supplement

I3C (Indole-3-Carbinol) is a phytochemical found in cruciferous vegetables (broccoli, cabbage, cauliflower, Brussels sprouts). Under stomach acid conditions, I3C polymerizes to form DIM (Diindolylmethane) and other indole compounds - the actual bioactive metabolites in vivo. This is why DIM supplementation is often preferred (more direct delivery of the active compound).

Quick Start

Route

Oral capsule

Start low

200-400mg daily

Frequency

1-2x daily

Timing

With food (acid environment supports DIM conversion)

With meals.

❋

Start here. Every protocol below is ordered lowest-first. Begin at the smallest effective dose, hold about a week to assess tolerance, and step up only if needed.

Tiered Protocols · lowest first

Quick Start

Conservative start

200-400mg daily · Oral capsule · 1-2x daily

Lowest starting point. Hold about a week to assess tolerance before stepping up.

200-400mg daily

1-2x daily

Standard

Estrogen Metabolism

200 mg · Oral · 2x Daily

human study · Michnovicz et al. 1997 (J Natl Cancer Inst)

200 mg

2x Daily

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How it works

I3C and its in vivo metabolites act via:

Stomach Acid Polymerization

I3C is unstable in stomach acid - polymerizes to DIM and other indole compounds. DIM is the active form.

CYP1A1 Induction

Shifts estrogen metabolism toward 2-hydroxyestrone (less proliferative) over 16α-hydroxyestrone (more proliferative). Theoretical benefit in estrogen-driven conditions.

Aryl Hydrocarbon Receptor (AhR) Modulation

AhR ligand activity drives Phase 1/2 detox enzyme upregulation.

Anti-Estrogenic at Cellular Level

Modulates estrogen receptor activity beyond simple metabolism shift.

Cervical Dysplasia

Multiple trials show I3C supplementation helps regression of cervical dysplasia (CIN II/III).

What to expect

Early

Days 1–7

Days 1-14: subtle effects.

Mid

Weeks 2–4

Weeks 4-12: peak metabolism shift.

Later

Weeks 4–12

Long-term continuous use OK.

Evidence

HumanPresent

AnimalStrong

In-vitroPresent

2000Indole-3-carbinol in the treatment of CIN · Bell MC et al, Gynecologic Oncology ↗peer reviewed

1991Indole-3-carbinol and estrogen metabolism · Bradlow HL et al, Carcinogenesis ↗peer reviewed

2007Cruciferous vegetables and human cancer prevention · Higdon JV et al, Pharmacological Research ↗review

Interactions & stacking

What's safe to combine, and what belongs in a separate pin.

Keep separate

Side effects & safety

Commonly reported

Mild GI
Mild headache (rare)

Less common & notes

CYP enzyme induction at high doses.
Allergic reactions rare.
Long-term safety good at standard doses.

Pharmacokinetics

Illustrative plasma concentration · 24 h

Half-life

Polymerized DIM ~hours

Peak · Tmax

1-3 hours

Elimination

Cumulative effects over weeks

Activity

Daily dosing for sustained effects

Storage. Capsule. Cool dry location.

Regulatory status

Dietary supplement (US, DSHEA).

Put it to work

Calculate, log & track

Open I3C straight into OnePin with your vial and dose pre-filled, then let it handle the syringe math and remind you before the vial runs out.

Open I3C in the app →

Go deeper

The guide & community

The complete I3C walkthrough, real protocol discussion and coaching live inside the BlessUp community on Skool.

Join the community ↗

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