OnePin Peptide Library · Mitochondrial
Humanin
HN Peptide · Humanin S14G
Humanin is a 24-amino acid mitochondrial-derived peptide (MDP) encoded by the 16S rRNA gene of mitochondrial DNA. It was originally discovered in 2001 from surviving neurons in Alzheimer's disease brain tissue, suggesting a role in cellular survival under stress. Humanin is cytoprotective, anti-apoptotic, and neuroprotective, activating the STAT3 signaling pathway through a trimeric receptor complex (CNTFR/WSX-1/gp130). Along with MOTS-c, Humanin represents a class of signaling peptides that communicate mitochondrial health status to the rest of the cell and to distant tissues. Humanin levels decline with age, correlating with increased cellular vulnerability.
Any time of day. No fasting required. Mitochondrial-derived peptide.
Start here. Every protocol below is ordered lowest-first. Begin at the smallest effective dose, hold about a week to assess tolerance, and step up only if needed.
How it works
Humanin binds to a trimeric receptor complex (CNTFR/WSX-1/gp130), activating JAK-STAT3 signaling which upregulates anti-apoptotic genes (Bcl-2 family) and suppresses pro-apoptotic signals (Bax, caspases). It also binds intracellularly to IGFBP-3 and Bax, directly blocking apoptotic cascade initiation. Humanin provides cellular stress resistance by maintaining mitochondrial membrane potential, reducing ROS production, and preventing cytochrome c release. As an MDP, it functions as a retrograde signal from mitochondria to nucleus, communicating mitochondrial health status and activating protective gene expression programs.
Humanin binds to a trimeric receptor complex (CNTFR/WSX-1/gp130), activating JAK-STAT3 signaling which upregulates anti-apoptotic genes (Bcl-2 family) and suppresses pro-apoptotic signals (Bax, caspases). It also binds intracellularly to IGFBP-3 and Bax, directly blocking apoptotic cascade initiation. Humanin provides cellular stress resistance by maintaining mitochondrial membrane potential, reducing ROS production, and preventing cytochrome c release. As an MDP, it functions as a retrograde signal from mitochondria to nucleus, communicating mitochondrial health status and activating protective gene expression programs.
What to expect
Days 1-14: No perceptible changes. Cellular signaling initiated.
Weeks 2-6: Cumulative protective effects.
Weeks 6+: Long-term cellular resilience. Best assessed through biomarkers.
Evidence
The honest take
Neuroprotection in Alzheimer's
Originally discovered in surviving AD neurons. Animal models show protection. Not a validated AD treatment.
Mechanistically confirmed. STAT3 and Bax binding well-characterized. Direct cellular protection.
Interactions & stacking
What's safe to combine, and what belongs in a separate pin.
Safe & synergistic
Keep separate
Side effects & safety
Commonly reported
- Well tolerated (endogenous peptide)
- Mild injection site irritation
Less common & notes
- Very limited adverse event data.
- Endogenous peptide suggesting good tolerability.
- Long-term data not available.
Pharmacokinetics
Storage. Standard BAC water 2-8C. Stable for 28 days. Protect from light.
Regulatory status
Not FDA-approved. Available as research peptide. WADA status not listed.
Put it to work
Calculate, log & track
Open Humanin straight into OnePin with your vial and dose pre-filled, then let it handle the syringe math and remind you before the vial runs out.
Open Humanin in the app →Go deeper
The guide & community
The complete Humanin walkthrough, real protocol discussion and coaching live inside the BlessUp community on Skool.
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