Mitochondrial Mitochondrial-derived peptide (MDP)Not FDA-approved · research compound

Humanin

HN Peptide · Humanin S14G

Evidence: Studied in humans. Example dose: finalize with a provider. Not a fact-check stamp.

1-5 mg daily – 1-2 mg SubQ Daily or specific days

Cytoprotective / anti-apoptotic peptideSTAT3 pathway activatorNeuroprotective signaling peptide

Humanin is a 24-amino acid mitochondrial-derived peptide (MDP) encoded by the 16S rRNA gene of mitochondrial DNA. It was originally discovered in 2001 from surviving neurons in Alzheimer's disease brain tissue, suggesting a role in cellular survival under stress. Humanin is cytoprotective, anti-apoptotic, and neuroprotective, activating the STAT3 signaling pathway through a trimeric receptor complex (CNTFR/WSX-1/gp130). Along with MOTS-c, Humanin represents a class of signaling peptides that communicate mitochondrial health status to the rest of the cell and to distant tissues. Humanin levels decline with age, correlating with increased cellular vulnerability.

Quick Start

Route

SubQ injection

Start low

1-5 mg SubQ

Frequency

Daily or specific days

Timing

No fasting required

Any time of day. No fasting required. Mitochondrial-derived peptide.

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Start here. Every protocol below is ordered lowest-first. Begin at the smallest effective dose, hold about a week to assess tolerance, and step up only if needed.

Tiered Protocols · lowest first

Conservative start

1-5 mg daily · SubQ injection · Daily or specific days

conservative starter

1-5 mg daily

Daily or specific days

Intermediate

Mitochondrial Derived Protection

1-2 mg · SubQ · Every other day

human study · Yen et al. 2013 (J Mol Endocrinol, Vol 50 Issue 1)

Often cycled alongside or alternated with MOTS-c for comprehensive mitochondrial support.

1-2 mg

Every other day

Reconstitution CalculatorU-100

Vial strength

BAC water

Vials in this mix

Syringe size

Target dose

050100u

Draw to

— units

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I will split this dose across more than one syringe

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How it works

Humanin binds to a trimeric receptor complex (CNTFR/WSX-1/gp130), activating JAK-STAT3 signaling which upregulates anti-apoptotic genes (Bcl-2 family) and suppresses pro-apoptotic signals (Bax, caspases). It also binds intracellularly to IGFBP-3 and Bax, directly blocking apoptotic cascade initiation. Humanin provides cellular stress resistance by maintaining mitochondrial membrane potential, reducing ROS production, and preventing cytochrome c release. As an MDP, it functions as a retrograde signal from mitochondria to nucleus, communicating mitochondrial health status and activating protective gene expression programs.

What to expect

Early

Days 1–7

Days 1-14: No perceptible changes. Cellular signaling initiated.

Mid

Weeks 2–4

Weeks 2-6: Cumulative protective effects.

Later

Weeks 4–12

Weeks 6+: Long-term cellular resilience. Best assessed through biomarkers.

Evidence

HumanPresent

AnimalModerate

In-vitroPresent

Each reference below was re-fetched from its PubMed record, and the quoted sentence was confirmed to appear in that abstract and to support the claim it sits under. Follow any of them to read the source.

Evidence boundary from the checked abstract: Furthermore, both humanin and MOTS-c improve insulin sensitivity in mouse models of type 2 diabetes.

Furthermore, both humanin and MOTS-c improve insulin sensitivity in mouse models of type 2 diabetes.

Mitochondrial-Derived Peptides Exacerbate Senescence. Rejuvenation research · 2018 · PMID 30058454

Evidence scope. This entry supports only the exact statement quoted from the live abstract; it does not independently verify OnePin's displayed dose, route, frequency, formulation, population, or broader mechanism.

Claims we could not support

No abstract we checked supports these for this molecule at this route. That is not the same as saying they are false — it marks where the evidence is missing.

Dose/route: 1-5 mg daily; SubQ injection; Daily or specific days. No checked live PubMed abstract verified this exact displayed dose, route, and frequency for the same molecule/formulation and relevant population.

Primary safety claim: No primary safety text present in source JSON. No checked live PubMed abstract directly verified this source-JSON safety claim at the displayed formulation, route, and regimen.

The honest take

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Neuroprotection in Alzheimer's

Originally discovered in surviving AD neurons. Animal models show protection. Not a validated AD treatment.

Anti-apoptotic

Mechanistically confirmed. STAT3 and Bax binding well-characterized. Direct cellular protection.

Interactions & stacking

Two different questions: what is documented about running these together, and what is documented about drawing them into the same syringe. A good pharmacological partner can still have to be pinned separately.

Documented together

BPC-157Non-overlapping mechanisms

GHK-CuNon-overlapping mechanisms

GHKNon-overlapping mechanisms

SemaxHumanin neuroprotection + Semax cognitive

Keep separate

CJC-1295 with DACDAC maleimide reactivity.

Check any specific pair in the Stack & Interaction Checker, which answers both questions separately. Absence of a documented conflict is not evidence of safety.

Side effects & safety

Commonly reported

Well tolerated (endogenous peptide)
Mild injection site irritation

Less common & notes

Very limited adverse event data.
Endogenous peptide suggesting good tolerability.
Long-term data not available.

Pharmacokinetics

Illustrative plasma concentration · 2.3 h

Half-life

0.5h

Peak · Tmax

0.25h

Elimination

2.5h

Bioavailability

~100%

SubQ

Duration of action

6-12 hours

Cytoprotective signaling

Clearance

Enzymatic degradation (proteases)

Storage. Standard BAC water 2-8C. Stable for 28 days. Protect from light.

Regulatory status

Not FDA-approved. Available as research peptide. WADA status not listed.

Put it to work

Calculate, log & track

Open Humanin straight into OnePin with your vial and dose pre-filled, then let it handle the syringe math and remind you before the vial runs out.

Open Humanin in the app →

Go deeper

The guide & community

The complete Humanin walkthrough, real protocol discussion and coaching live inside the BlessUp community on Skool.

Join the community ↗

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