library.onepin.app/cjc-1295-no-dac Peptide Education Library
Education only, not medical advice 21+ Every dose is an example to finalize with a licensed provider
Growth Hormone Synthetic GHRH analogNot FDA-approved · research compound

CJC-1295 no DAC

Mod GRF 1-29 · Modified GRF (1-29) · ModGRF1-29

100 mcg per injection – 100 mcg per dose SubQ 1-3 times daily
Modified GRF(1-29)Growth hormone secretagogue29-amino acid peptide with 4 substitutions

CJC-1295 without DAC (also known as Modified GRF 1-29 or Mod GRF) is a synthetic 29-amino acid peptide analog of growth hormone-releasing hormone (GHRH). It consists of the first 29 amino acids of endogenous GHRH with four amino acid substitutions (positions 2, 8, 15, 27) that protect against enzymatic degradation while preserving full biological activity at the GHRH receptor. Unlike CJC-1295 with DAC, this variant does NOT contain the Drug Affinity Complex (maleimide linker) that provides albumin binding, resulting in a shorter half-life of approximately 30 minutes versus 6-8 days. This shorter duration preserves natural pulsatile GH release patterns. It is the most commonly paired GHRH analog with Ipamorelin, sold as a pre-mixed blend by many compounding pharmacies.

Quick Start
Route
SubQ injection
Start low
100 mcg SubQ
Frequency
1-3 times daily
Timing
Fasted. 2+ hours empty stomach for optimal GH response.

Starting dose. 100 mcg — Per injection

Same fasting rules as Ipamorelin. Administer together with Ipamorelin at bedtime on empty stomach. 2+ hours fasted.

Start here. Every protocol below is ordered lowest-first. Begin at the smallest effective dose, hold about a week to assess tolerance, and step up only if needed.

Tiered Protocols · lowest first
Conservative start
100 mcg per injection · SubQ injection · 1-3 times daily
conservative starter
100 mcg per injection
1-3 times daily
Standard
Anti-Aging Pulse
100 mcg · SubQ · Daily
anecdotal · community
Also called Mod GRF 1-29; ~30-minute half-life, dosed near a GH pulse and almost always stacked with a GHRP (e.g. Ipamorelin). The published CJC-1295 PK trial (Teichman 2006) used the DAC version, not this one.
100 mcg
Daily
Intermediate
Athletic (Multi-Dose)
100 mcg per dose · SubQ · 3x Daily
anecdotal · community
Multiple daily fasted pulses, each with a GHRP. Strict fasting (2h before, 30 min after).
100 mcg per dose
3x Daily
Reconstitution CalculatorU-100
050100u
Draw to
units
01

How it works

CJC-1295 no DAC binds to GHRH receptors on pituitary somatotrophs, activating cAMP/PKA signaling to release stored GH granules. The four amino acid substitutions (Ala2->D-Ala, Asn8->Gln, Ala15->Ala(modified), Met27->Leu) protect against DPP-IV degradation and methionine oxidation. Without the DAC maleimide linker, it does not bind albumin and is cleared rapidly (~30 min half-life), producing a single acute GH pulse that mimics natural pulsatile secretion. When paired with Ipamorelin (ghrelin receptor agonist), the dual GHRH + GHRP stimulation amplifies pulse amplitude and frequency, producing 3-5x greater GH release than either peptide alone.

02

What to expect

Early
Days 1–7

Days 1-7: Improved sleep. Mild flushing post-injection. No visible body composition changes yet.

Mid
Weeks 2–4

Weeks 2-6: Recovery improvements. Sleep consistently better. Body comp changes beginning.

Later
Weeks 4–12

Weeks 6-16: Significant body composition changes when paired with Ipamorelin, diet, and training.

03

Evidence

HumanModerate
AnimalPresent
In-vitroPresent

Claims we could not support

No abstract we checked supports these for this molecule at this route. That is not the same as saying they are false — it marks where the evidence is missing.

Dose/route: 100 mcg SubQ per injection 1-3 times daily while fasted. No checked human abstract administered exact CJC-1295 without DAC (modified GRF 1-29) at this regimen. Human CJC-1295 trials located for the source record used the DAC/long-acting molecule and cannot be transferred.

Mechanism/PK: exact no-DAC molecule, ~30-minute half-life, and acute GH pulse. The only exact “modified GRF 1-29” PubMed hit was an analytical doping-material paper, not a human mechanistic or pharmacokinetic study. DAC-CJC-1295 evidence was rejected as a different formulation.

Primary safety concern at the displayed SubQ regimen. No checked exact-molecule human safety abstract was found.

04

The honest take

Pulsatile vs continuous GH

Short half-life (~30 min) preserves natural GH pulsatility, which is considered superior to continuous GH elevation for body composition and health. This is its advantage over CJC-1295 with DAC.

Most popular GH peptide stack

CJC no DAC + Ipamorelin is the most widely used GH peptide combination in the community. Sold pre-mixed by numerous compounding pharmacies.

05

Interactions & stacking

Two different questions: what is documented about running these together, and what is documented about drawing them into the same syringe. A good pharmacological partner can still have to be pinned separately.

Documented together

BPC-157BPC upregulates GH receptors, amplifying CJC effects
VIPNo chemical conflict. Complementary pathways.
GHRP-2GHRH + GHRP synergy
GHRP-6GHRH + GHRP synergy

Keep separate

TesamorelinSame GHRH receptor target. Redundant stimulation with no added benefit.
CJC-1295 with DACDo not run both CJC variants simultaneously.

Check any specific pair in the Stack & Interaction Checker, which answers both questions separately. Absence of a documented conflict is not evidence of safety.

06

Side effects & safety

Commonly reported

  • Transient flushing post-injection
  • Mild water retention first 1-2 weeks
  • Occasional headache

Less common & notes

  • Joint stiffness from GH/IGF-1 elevation.
  • Monitor fasting glucose.
  • Contraindicated in active malignancy.
07

Pharmacokinetics

Illustrative plasma concentration · 2.3 h
Half-life
0.5h
Peak · Tmax
0.25h
Elimination
2.5h
Bioavailability
~100%

SubQ

Duration of action
2-3 hours

Acute GH pulse; GH/IGF-1 elevation lasts several hours post-injection

Clearance

Enzymatic degradation (DPP-IV and other peptidases); renal excretion

Storage. Shorter stability than most peptides. Use within 14-21 days after reconstitution. DPP-IV degradation continues in solution despite amino acid substitutions. Refrigerate 2-8C. This shelf life becomes limiting factor for CJC/Ipa pre-mixed blends.

08

Regulatory status

Not FDA-approved. WADA prohibited under S2. Available through compounding and research channels.

Put it to work

Calculate, log & track

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Go deeper

The guide & community

The complete CJC-1295 no DAC walkthrough, real protocol discussion and coaching live inside the BlessUp community on Skool.

Join the community ↗
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