OnePin Peptide Library · Growth Hormone

Growth Hormone Growth Hormone Secretagogue Blend Not FDA-approved · investigational

CJC-1295 no DAC / GHRP-6 Blend

CJC + GHRP-6

Growth Hormone Secretagogue BlendGHRH + GHRP CombinationStrong appetite-stimulating GH blend29-Amino Acid Analog + Synthetic Hexapeptide

CJC-1295 no DAC / GHRP-6 Blend is a pre-mixed growth hormone secretagogue combination that pairs Modified GRF(1-29) with the original and most appetite-stimulating GHRP. This blend produces powerful pulsatile GH release through dual-receptor activation while delivering the strongest hunger drive of any GH peptide stack. GHRP-6 was the first synthetic ghrelin mimetic developed and remains the go-to GHRP for users who need or want significant appetite stimulation alongside GH optimization.

Quick Start

Route

Subcutaneous (SubQ) injection

Start low

100mcg CJC + 100mcg GHRP-6 per injection

Frequency

2-3x daily

Timing

Fasted. Minimum 2 hours empty stomach. No eating for 30 min post-injection. Use the hunger window strategically for your largest meal.

❋

Start here. Every protocol below is ordered lowest-first. Begin at the smallest effective dose, hold about a week to assess tolerance, and step up only if needed.

Tiered Protocols · lowest first

Quick Start

Conservative start

100mcg CJC + 100mcg GHRP-6 per injection · Subcutaneous (SubQ) injection · 2-3x daily

Lowest starting point. Hold about a week to assess tolerance before stepping up.

100mcg CJC + 100mcg GHRP-6 per injection

2-3x daily

Standard

Mass / Appetite

~200 mcg total (100/100) · SubQ · 2x Daily

anecdotal · community

~200 mcg total (100/100)

2x Daily

Reconstitution CalculatorU-100

Vial strength

BAC water

Target dose

050100u

Draw to

— units

◆

How it works

This blend works through dual-receptor stimulation of the GH axis with pronounced ghrelin-mediated appetite effects:

CJC-1295 no DAC (Mod GRF 1-29)

Binds GHRH receptors on pituitary somatotrophs, activating the cAMP/PKA signaling cascade to prime and release stored GH granules. The four amino acid substitutions protect against DPP-IV degradation, extending the half-life to approximately 30 minutes. Produces acute GH pulses preserving natural pulsatile patterns.

GHRP-6

Binds GHS-R1a (ghrelin receptor) on the pituitary with strong efficacy, triggering GH release through the PLC/IP3/PKC pathway. GHRP-6 has the strongest orexigenic effect of any GHRP because it activates hypothalamic appetite centers more potently than GHRP-2 or Ipamorelin. It also suppresses somatostatin, removing the natural brake on GH secretion. GHRP-6 elevates cortisol and prolactin in a dose-dependent manner - more than Ipamorelin, less than Hexarelin.

Synergistic Amplification

The simultaneous GHRH signal (CJC) and GHRP signal (GHRP-6) converge through independent intracellular pathways, producing GH pulses 3-5x greater than either peptide alone. This mimics the body's natural dual-signal GH regulation system.

Appetite Mechanism

GHRP-6 activates neuropeptide Y (NPY) and agouti-related peptide (AgRP) neurons in the arcuate nucleus of the hypothalamus, triggering strong hunger signaling within 15-20 minutes of injection. This is the same pathway activated by endogenous ghrelin before meals.

What to expect

Early

Days 1–7

Days 1-7: Strong appetite stimulation is the first and most obvious effect, peaking 15-20 minutes post-injection. Use this hunger window to time your largest meals. Improved sleep quality with deeper slow-wave sleep, especially with bedtime dosing. Possible mild water retention. Transient flushing or tingling post-injection is normal.

Mid

Weeks 2–4

Weeks 2-6: Body composition changes visible with adequate caloric intake. Muscle fullness and recovery improving. Skin quality starting to improve. Appetite effect becomes predictable and can be strategically timed around training and meals. Fat distribution may shift as GH levels optimize.

Later

Weeks 4–12

Weeks 6-12: Full body recomposition effects when paired with training and nutrition. Lean mass gains consolidated. Skin, hair, and connective tissue improvements visible. Consider cycling off after 8-12 weeks with a 4-week break to maintain pituitary sensitivity and prevent GHS-R1a desensitization.

Evidence

HumanStrong

AnimalPresent

In-vitroStrong

1992The growth hormone-releasing activity of a synthetic hexapeptide in normal men and short statured children after oral administration · Bowers CY et al, Journal of Clinical Endocrinology & Metabolism ↗peer reviewed

2017Synthetic Growth Hormone-Releasing Peptides (GHRPs): A Historical Appraisal of the Evidences Supporting Their Cytoprotective Effects · Berlanga-Acosta J et al, Cardiovascular & Hematological Disorders - Drug Targets ↗review

2006Prolonged stimulation of growth hormone (GH) and insulin-like growth factor I secretion by CJC-1295, a long-acting analog of GH-releasing hormone, in healthy adults · Teichman SL et al, Journal of Clinical Endocrinology & Metabolism ↗peer reviewed

1996Growth hormone-releasing peptides: clinical and basic aspects · Bowers CY, Hormone Research ↗review

The honest take

◆

Strongest appetite stimulation of any GH peptide blend

Supported

Well-supported by human comparative data. GHRP-6 consistently produces the most intense hunger response among GHRPs, peaking at 15-20 minutes post-injection. This is due to stronger hypothalamic NPY/AgRP activation compared to GHRP-2 or Ipamorelin.

3-5x synergistic GH amplification from dual-receptor stimulation

Supported by GHRH + GHRP co-administration studies. The synergistic effect is well-established in the literature, though exact magnitude varies by individual and dose.

Strategic meal timing using post-injection hunger window

Practically validated. The 15-20 minute onset and 30-60 minute duration of appetite stimulation is consistent across users, making it predictable enough for meal timing strategies.

Interactions & stacking

What's safe to combine, and what belongs in a separate pin.

Safe & synergistic

AOD-9604No interaction. GH fragment peptide works through different pathway. Safe in same syringe.

GHK-CuCompatible for stacking. Draw GHK-Cu LAST due to copper. GH elevation enhances collagen remodeling.

MOTS-cMitochondrial peptide with independent mechanism. Safe in same syringe.

SelankNo reactive residue conflicts. Safe in same syringe.

Keep separate

CJC-1295 with DACDAC version provides continuous GH elevation that disrupts pulsatile signaling. Do not combine sustained and pulsatile GHRH analogs.

Somatostatin analogs (Octreotide)Directly antagonizes GH release. Completely negates the purpose of this blend.

High-dose insulinElevated insulin blunts GH response. Fasted state is required for optimal GH release from this blend.

NAD+Acidic pH of NAD+ can denature peptides. Different route anyway (NAD+ is typically IM or IV).

Side effects & safety

Commonly reported

Strong appetite increase 15-20 min post-injection lasting 30-60 min
Transient flushing or warmth post-injection (resolves in minutes)
Mild tingling or head rush immediately after injection
Mild water retention in first 1-2 weeks
Vivid dreams and improved sleep (desired effect)

Less common & notes

At higher GHRP-6 doses (above 200mcg), cortisol and prolactin elevation becomes more significant.
This can manifest as mild anxiety, irritability, or lethargy post-injection.
GHRP-6 has the strongest appetite effect of any GHRP - some users find the hunger unmanageable if not strategically planned.
Occasional stomach gurgling or GI discomfort from the ghrelin activation.
Keep individual GHRP-6 doses at or below 200mcg to balance GH output against hormonal side effects.
Both components have reasonable safety data, but long-term human combination studies are limited.

Pharmacokinetics

Illustrative plasma concentration · 24 h

Half-life

CJC-1295 no DAC: ~30 min; GHRP-6: ~20 min. Combined GH pulse duration: 1.5-2 hours.

Peak · Tmax

CJC: 15-30 min; GHRP-6: 15-20 min. Peak synergistic GH release at ~20-30 min post-injection.

Elimination

Both components cleared within 2-3 hours. GH pulse elevation returns to baseline by 3-4 hours.

Activity

GH pulse lasts 1.5-2 hours. Downstream IGF-1 elevation persists 12-20 hours. Appetite effect from GHRP-6 lasts 30-60 minutes post-injection, peaking at 15-20 minutes.

Storage. Store lyophilized vial at room temperature or refrigerated. After reconstitution with bacteriostatic water, store refrigerated at 2-8 degrees C. Use within 28-30 days of reconstitution. Do not freeze after reconstitution. Protect from prolonged light exposure. Discard if solution becomes cloudy or contains particulate matter.

Regulatory status

Not FDA-approved as a combination product. Available through compounding pharmacies with prescription. CJC-1295 no DAC (Mod GRF 1-29) has published human pharmacokinetic data. GHRP-6 has been studied in multiple human clinical trials for GH release and diagnostic purposes. Pre-mixed blends are a compounding pharmacy product. Classified as research peptides in most jurisdictions. All use is experimental and off-label.

Put it to work

Calculate, log & track

Open CJC-1295 no DAC / GHRP-6 Blend straight into OnePin with your vial and dose pre-filled, then let it handle the syringe math and remind you before the vial runs out.

Open CJC-1295 no DAC / GHRP-6 Blend in the app →

Go deeper

The guide & community

The complete CJC-1295 no DAC / GHRP-6 Blend walkthrough, real protocol discussion and coaching live inside the BlessUp community on Skool.

Join the community ↗

Open in app Community