library.onepin.app/thymosin-beta-4-full Peptide Education Library
Education only, not medical advice 21+ Every dose is an example to finalize with a licensed provider

OnePin Peptide Library · Repair & Recovery

Repair & Recovery Peptide Not FDA-approved · investigational

Thymosin Beta-4 Full

TB4 Full · Thymosin Beta-4 Full Length

Thymosin Beta-4 (TB4) is a naturally occurring 43-amino acid peptide that is the most abundant member of the beta-thymosin family. Originally isolated from the thymus gland by Allan Goldstein in the 1960s-70s, TB4 is now known to be expressed in virtually all nucleated cells throughout the body. It is the primary intracellular G-actin sequestering peptide, playing a fundamental role in actin cytoskeleton dynamics, cell motility, and tissue repair.,Unlike its truncated fragment TB-500 (which contains the active 17-amino acid sequence Ac-SDKP and surrounding residues), full-length Thymosin Beta-4 includes the complete native protein with all of its biological domains. TB4 promotes wound healing through multiple mechanisms including cell migration, angiogenesis, reduction of inflammation, and prevention of apoptosis. It also has cardioprotective properties and promotes hair follicle growth.,Thymosin Beta-4 is not currently FDA-approved for any indication, though it has been the subject of clinical trials for wound healing (particularly corneal wounds and cardiac repair after myocardial infarction). RegeneRx Biopharmaceuticals developed RGN-259 (TB4 eye drops) which showed positive Phase II results for neurotrophic keratopathy. The full-length peptide is available as a research compound.

Quick Start
Route
Subcutaneous (SubQ)
Start low
750mcg
Frequency
2x/week
Timing
No specific requirement

Same timing as TB-500. Any time of day, no fasting required.

Start here. Every protocol below is ordered lowest-first. Begin at the smallest effective dose, hold about a week to assess tolerance, and step up only if needed.

Tiered Protocols · lowest first
Quick Start
Conservative start
750mcg · Subcutaneous (SubQ) · 2x/week
Lowest starting point. Hold about a week to assess tolerance before stepping up.
750mcg
2x/week
Intermediate
Full Chain Systemic Repair
2.5 mg · SubQ · 2x Weekly
animal study · Smart et al. 2007 (Nature)
2.5 mg
2x Weekly
Reconstitution CalculatorU-100
050100u
Draw to
units
01

How it works

Sequesters G-actin monomers to regulate actin polymerization, controlling cytoskeletal dynamics essential for cell migration, wound closure, and tissue remodeling. The N-terminal tetrapeptide Ac-SDKP has potent anti-inflammatory and anti-fibrotic properties.,Promotes angiogenesis by upregulating VEGF and stimulating endothelial cell migration and tube formation. Also activates cardiac progenitor cells and promotes cardiomyocyte survival after ischemic injury via Akt/PI3K signaling pathway.,Reduces inflammation by downregulating NF-kB-mediated inflammatory cytokines (IL-1beta, IL-6, TNF-alpha) and promotes anti-inflammatory macrophage (M2) polarization. Inhibits apoptosis in damaged cells via activation of survival signaling pathways.

Sequesters G-actin monomers to regulate actin polymerization, controlling cytoskeletal dynamics essential for cell migration, wound closure, and tissue remodeling. The N-terminal tetrapeptide Ac-SDKP has potent anti-inflammatory and anti-fibrotic properties.,Promotes angiogenesis by upregulating VEGF and stimulating endothelial cell migration and tube formation. Also activates cardiac progenitor cells and promotes cardiomyocyte survival after ischemic injury via Akt/PI3K signaling pathway.,Reduces inflammation by downregulating NF-kB-mediated inflammatory cytokines (IL-1beta, IL-6, TNF-alpha) and promotes anti-inflammatory macrophage (M2) polarization. Inhibits apoptosis in damaged cells via activation of survival signaling pathways.

02

What to expect

Early
Days 1–7

Days 1-7: Anti-inflammatory effects begin. Reduced pain and swelling at injury sites. Cellular migration and early angiogenesis initiated.

03

Evidence

HumanPresent
AnimalStrong
In-vitroPresent
1999Thymosin beta4 accelerates wound healing · Malinda KM et al, Journal of Investigative Dermatology ↗peer reviewed
2012Thymosin beta4: a multi-functional regenerative peptide. Basic properties and clinical applications · Goldstein AL et al, Expert Opinion on Biological Therapy ↗review
2007Development of thymosin beta4 for treatment of patients with ischemic heart disease · Crockford D et al, Annals of the New York Academy of Sciences ↗review
2010A randomized, placebo-controlled, single and multiple dose study of intravenous thymosin beta4 in healthy volunteers · Ruff D et al, Annals of the New York Academy of Sciences ↗peer reviewed
04

Interactions & stacking

What's safe to combine, and what belongs in a separate pin.

Safe & synergistic

Thymosin Alpha-1Different thymosin family members - TB4 for tissue repair, TA-1 for immune modulation. Non-conflicting
IpamorelinGH secretagogue and tissue repair peptide support recovery through independent pathways
N-Acetyl Selank AmidateAnxiolytic peptide and tissue repair peptide have no pharmacological interaction
KPVAnti-inflammatory tripeptide and tissue repair peptide address different aspects of healing

Keep separate

GHK-CuCopper-peptide can oxidize methionine residue in TB4 full-length sequence, potentially reducing bioactivity - do not mix in same syringe
05

Pharmacokinetics

Illustrative plasma concentration · 24 h
Half-life
~2-3 hours
Peak · Tmax
~30-60 min SubQ
Elimination
~10-15 hours
Activity
4-7 days (actin regulation, cell migration, and tissue repair effects persist well beyond plasma clearance)

Storage. Store refrigerated at 2-8C. Reconstituted solution should be used within 14-28 days. Protect from light.

06

Regulatory status

Not FDA-approved. Investigational new drug for corneal wound healing (RGN-259). Research compound for all other applications. WADA prohibited substance.

Put it to work

Calculate, log & track

Open Thymosin Beta-4 Full straight into OnePin with your vial and dose pre-filled, then let it handle the syringe math and remind you before the vial runs out.

Open Thymosin Beta-4 Full in the app →

Go deeper

The guide & community

The complete Thymosin Beta-4 Full walkthrough, real protocol discussion and coaching live inside the BlessUp community on Skool.

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