library.onepin.app/mgf Peptide Education Library
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OnePin Peptide Library · Repair & Recovery

Repair & Recovery Peptide Not FDA-approved · investigational

MGF

Mechano Growth Factor · IGF-1Ec

Mechano Growth Factor (MGF) is a splice variant of the IGF-1 gene (IGF-1Ec in humans, IGF-1Eb in rodents) that is produced locally in muscle tissue in response to mechanical stress, such as resistance exercise or tissue damage. Unlike systemic IGF-1, MGF is expressed transiently and acts in an autocrine/paracrine manner at the site of muscle damage. It plays a key role in activating muscle satellite (stem) cells, initiating the early repair and growth response following exercise-induced muscle damage.,The synthetic peptide form used in research is typically the 24-amino-acid C-terminal peptide of the MGF E-domain, often PEGylated (PEG-MGF) to extend its extremely short biological half-life. Native MGF has a half-life of only 5-7 minutes due to rapid enzymatic degradation, while PEGylation extends this to several hours. MGF differs from mature IGF-1 in that it preferentially activates satellite cell proliferation (early muscle repair) rather than differentiation, making it complementary to IGF-1 in the muscle regeneration timeline.,MGF has not been approved for human use by any regulatory agency and remains a research compound. Most of the scientific literature on MGF consists of basic science studies on its role in muscle biology, cardiac repair, and neuroregeneration. While the endogenous biology of MGF is well-characterized, the pharmacology of exogenous synthetic MGF peptide administration in humans is essentially unstudied in controlled clinical trials.

Quick Start
Route
Intramuscular (IM) - local injection into target muscle
Start low
100-200mcg
Frequency
Post-workout, 2-3x/week
Timing
No specific requirement

Post-workout injection at target muscle (IM). Pin alone. Inject within 30 minutes of training.

Start here. Every protocol below is ordered lowest-first. Begin at the smallest effective dose, hold about a week to assess tolerance, and step up only if needed.

Tiered Protocols · lowest first
Quick Start
Conservative start
100-200mcg · Intramuscular (IM) - local injection into target muscle · Post-workout, 2-3x/week
Lowest starting point. Hold about a week to assess tolerance before stepping up.
100-200mcg
Post-workout, 2-3x/week
Intermediate
Localized Hypertrophy
200 mcg · IM · 3x Weekly
human clinical trial · Hameed/Goldspink 2003 (J Physiol 555:231-40, PMID 14565994)
200 mcg
3x Weekly
Reconstitution CalculatorU-100
050100u
Draw to
units
01

How it works

MGF activates quiescent muscle satellite (stem) cells, causing them to re-enter the cell cycle and proliferate. This is a critical early step in muscle repair following damage. MGF acts via a distinct receptor pathway from mature IGF-1, primarily activating satellite cell proliferation rather than differentiation.,Signals through the IGF-1 receptor but with different downstream effects than mature IGF-1, preferentially activating the ERK1/2 (proliferative) pathway over the PI3K/Akt (differentiation/hypertrophy) pathway. This makes MGF an early-phase repair signal that precedes the later IGF-1-driven hypertrophy response.,Also inhibits satellite cell differentiation during the proliferative phase, ensuring an adequate pool of progenitor cells is generated before they commit to becoming mature muscle fibers. This temporal regulation is essential for optimal muscle repair and may support hyperplasia (new fiber formation).

MGF activates quiescent muscle satellite (stem) cells, causing them to re-enter the cell cycle and proliferate. This is a critical early step in muscle repair following damage. MGF acts via a distinct receptor pathway from mature IGF-1, primarily activating satellite cell proliferation rather than differentiation.,Signals through the IGF-1 receptor but with different downstream effects than mature IGF-1, preferentially activating the ERK1/2 (proliferative) pathway over the PI3K/Akt (differentiation/hypertrophy) pathway. This makes MGF an early-phase repair signal that precedes the later IGF-1-driven hypertrophy response.,Also inhibits satellite cell differentiation during the proliferative phase, ensuring an adequate pool of progenitor cells is generated before they commit to becoming mature muscle fibers. This temporal regulation is essential for optimal muscle repair and may support hyperplasia (new fiber formation).

02

What to expect

Early
Days 1–7

Days 1-7: Satellite cell activation occurs within hours of injection (based on preclinical data). Subjective effects are minimal in the first week.

03

Evidence

HumanPresent
AnimalPresent
In-vitroStrong
2011Mechano Growth Factor E peptide (MGF-E), derived from an isoform of IGF-1, activates human muscle progenitor cells and induces an increase in their fusion potential at different ages · Kandalla PK et al, Mechanisms of Ageing and Development ↗peer reviewed
2010Minireview: Mechano-growth factor: a putative product of IGF-I gene expression involved in tissue repair and regeneration · Hill M, Goldspink G, Endocrinology ↗review
2012Mechano growth factor (MGF) promotes proliferation and inhibits differentiation of porcine satellite cells (PSCs) by down-regulation of key myogenic transcriptional factors · Yang H et al, Molecular and Cellular Biochemistry ↗peer reviewed
2006Impairment of IGF-I gene splicing and MGF expression associated with muscle wasting · Hameed M et al, International Journal of Biochemistry and Cell Biology ↗peer reviewed
04

Interactions & stacking

What's safe to combine, and what belongs in a separate pin.

Safe & synergistic

Testosterone CypionateAndrogens support the anabolic environment that complements MGF's satellite cell activation - no direct interaction
EpicatechinOral myostatin inhibitor complements injectable satellite cell activator - different mechanisms and routes
GHK-CuGHK-Cu supports tissue remodeling and repair - compatible with MGF's muscle-specific effects when injected at different sites
IGF-1 LR3Sequential mechanism - MGF activates satellite cell proliferation (early repair) then IGF-1 LR3 drives differentiation and hypertrophy (later growth phase)

Keep separate

Any compound in same syringeMGF is a fragile peptide with an extremely short half-life - must be injected alone directly into the target muscle, never mixed with other peptides
InsulinInsulin drives IGF-1 receptor signaling toward differentiation - may prematurely push MGF-activated satellite cells out of the proliferative phase, reducing the satellite cell pool
05

Pharmacokinetics

Illustrative plasma concentration · 24 h
Half-life
~5-7 min (native MGF), ~several hours (PEG-MGF)
Peak · Tmax
~5-10 min IM (native MGF), ~1-2 hours (PEG-MGF)
Elimination
~25-35 min (native MGF), ~12-24 hours (PEG-MGF)
Activity
30-60 minutes (native MGF), 6-12 hours (PEG-MGF); satellite cell activation persists beyond peptide clearance

Storage. Store refrigerated at 2-8C. Reconstituted solution should be used within 7-14 days. PEG-MGF is more stable than non-PEG form. Use bacteriostatic water for reconstitution.

06

Regulatory status

Not approved for human use by the FDA or any regulatory agency. Research chemical only. Listed as a prohibited substance by WADA (World Anti-Doping Agency).

Put it to work

Calculate, log & track

Open MGF straight into OnePin with your vial and dose pre-filled, then let it handle the syringe math and remind you before the vial runs out.

Open MGF in the app →

Go deeper

The guide & community

The complete MGF walkthrough, real protocol discussion and coaching live inside the BlessUp community on Skool.

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