library.onepin.app/orexin-a Peptide Education Library
Education only, not medical advice 21+ Every dose is an example to finalize with a licensed provider

OnePin Peptide Library · Cognitive & Mood

Cognitive & Mood Endogenous Hypothalamic Neuropeptide Not FDA-approved · investigational

Orexin A

Hypocretin-1 · OX-A

Endogenous Hypothalamic NeuropeptideOX1R/OX2R Dual AgonistInvestigational Narcolepsy TherapyResearch Chemical (no FDA approval)

Orexin A (also called Hypocretin-1) is a 33-amino-acid neuropeptide produced by lateral hypothalamic neurons. It is one of two endogenous orexin peptides (along with Orexin B / Hypocretin-2) that promote wakefulness, arousal, appetite, and energy expenditure via OX1R and OX2R receptors. Loss of orexin neurons causes narcolepsy with cataplexy in humans - the major clinical context for orexin pharmacology.

Quick Start
Route
Intranasal (primary) or SubQ
Start low
Per investigational protocol
Frequency
Variable
Timing
Independent

Intranasal preferred for CNS access. Reconstitute with bac water; refrigerate. Disulfide-containing - keep cold and protect from oxidation.

Start here. Every protocol below is ordered lowest-first. Begin at the smallest effective dose, hold about a week to assess tolerance, and step up only if needed.

Tiered Protocols · lowest first
Quick Start
Conservative start
Per investigational protocol · Intranasal (primary) or SubQ · Variable
Lowest starting point. Hold about a week to assess tolerance before stepping up.
Per investigational protocol
Variable
Expert
Narcolepsy/Wakefulness Intranasal
50-150 mcg · Nasal · As needed
animal study · Deadwyler et al. 2007 (J Neurosci)
50-150 mcg
As needed
Reconstitution CalculatorU-100
050100u
Draw to
units
01

How it works

Orexin A binds both orexin receptors (OX1R and OX2R) in approximately equal affinity, distinguishing it from Orexin B which is OX2R-selective:

OX1R Activation

Selectively expressed on dopaminergic VTA neurons and locus coeruleus norepinephrine neurons - drives reward-related behavior and arousal.

OX2R Activation

Expressed on tuberomammillary nucleus histaminergic neurons - drives wake-promotion. OX2R signaling is the primary wake-promoting orexin pathway (basis of OX2R-selective insomnia therapy with the antagonist class).

Wakefulness Maintenance

Endogenous orexin neurons fire during wake state and silence during sleep. Loss of these neurons (autoimmune destruction in narcolepsy with cataplexy) produces the disease. Exogenous Orexin A can theoretically substitute - main delivery challenge is CNS penetration.

Appetite / Feeding

Lateral hypothalamic orexin signaling stimulates feeding behavior - high-dose orexin administration produces hyperphagia.

Sympathetic Activation

Orexin signaling increases sympathetic tone - heart rate, blood pressure, energy expenditure all rise. Relevant to cardiovascular caution at high doses.

Disulfide-Containing

Two intramolecular disulfide bonds - chemistry-incompatible with copper-conjugated peptides like GHK-Cu.

02

What to expect

Early
Days 1–7

Acute wakefulness within 15-30 min of intranasal dose.

Mid
Weeks 2–4

Effects fade with plasma clearance.

Later
Weeks 4–12

No long-term cumulative effects characterized.

03

Evidence

HumanModerate
AnimalStrong
In-vitroPresent
2000Hypocretin-1 (orexin-A) deficiency in human narcolepsy · Nishino S et al, The Lancet ↗peer reviewed
2008Orexin A and orexin B - clinical and research applications · Baier PC et al, Sleep Medicine Reviews ↗review
2009Intranasal hypocretin-1 (orexin A) restores wakefulness in narcoleptic dogs · Schmidt S et al, Sleep ↗peer reviewed
2013Hypocretins (orexins) in narcolepsy and other sleep disorders · Sakurai T, Cell and Tissue Research ↗review
04

Interactions & stacking

What's safe to combine, and what belongs in a separate pin.

Keep separate

05

Side effects & safety

Commonly reported

  • Nasal irritation (intranasal)
  • Mild tachycardia
  • Possible insomnia at high doses or evening dosing

Less common & notes

  • Hypertension at high doses (sympathetic activation).
  • Anxiety or agitation.
  • Cardiovascular events theoretically possible at supraphysiologic doses.
  • Long-term safety not characterized in humans.
  • The intact orexin peptide has poor systemic distribution and the therapeutic window for non-narcolepsy use is unclear.
06

Pharmacokinetics

Illustrative plasma concentration · 24 h
Half-life
~30-60 min plasma
Peak · Tmax
15-30 min intranasal, longer SubQ
Elimination
1-3 hours wakefulness/arousal
Activity
1-3 hours per dose

Storage. Refrigerate. Light-sensitive. Reconstituted: 14-21 days. Disulfide-containing - keep cold.

07

Regulatory status

Not FDA-approved. Investigational status only. WADA: not specifically prohibited but may fall under stimulant regulations in sport contexts.

Put it to work

Calculate, log & track

Open Orexin A straight into OnePin with your vial and dose pre-filled, then let it handle the syringe math and remind you before the vial runs out.

Open Orexin A in the app →

Go deeper

The guide & community

The complete Orexin A walkthrough, real protocol discussion and coaching live inside the BlessUp community on Skool.

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