library.onepin.app/mt-i-melanotan-i Peptide Education Library
Education only, not medical advice 21+ Every dose is an example to finalize with a licensed provider

OnePin Peptide Library · Skin

Skin α-MSH Linear Analog ([Nle4, D-Phe7]) Not FDA-approved · investigational

MT-I (Melanotan I)

MT-1 · MT1 · Melanotan I · Afamelanotide · Scenesse

α-MSH Linear Analog ([Nle4, D-Phe7])MC1R-Selective Melanocortin AgonistFDA-Approved (Scenesse, 2019, for EPP)Off-Label Tanning Peptide

MT-I (Melanotan I, INN: afamelanotide, brand: Scenesse) is a synthetic linear analog of α-melanocyte-stimulating hormone (α-MSH) - a 13-amino-acid linear peptide with substitutions ([Nle4, D-Phe7]-α-MSH) that confer high MC1R receptor selectivity and increased metabolic stability. FDA-approved (October 2019) and EMA-approved as Scenesse for the prevention of phototoxicity in adult patients with erythropoietic protoporphyria (EPP) - a rare genetic disease causing severe sunlight-induced skin pain.

Quick Start
Route
Subcutaneous (SubQ) implant (FDA) or daily SubQ injection (off-label)
Start low
16 mg implant (FDA) or 0.5-1 mg daily (off-label)
Frequency
Every 2 months (FDA implant) or daily (off-label injection)
Timing
Timing independent of food

FDA Scenesse: 16 mg implant placed by clinician every 2 months. Off-label injection: 0.5-1 mg daily SubQ for 1-2 weeks during 'tan loading,' then 1-2x weekly maintenance. Reconstitute lyophilized peptide with bacteriostatic water; refrigerate. Light-sensitive after reconstitution.

Start here. Every protocol below is ordered lowest-first. Begin at the smallest effective dose, hold about a week to assess tolerance, and step up only if needed.

Tiered Protocols · lowest first
Quick Start
Conservative start
16 mg implant (FDA) or 0.5-1 mg daily (off-label) · Subcutaneous (SubQ) implant (FDA) or daily SubQ injection (off-label) · Every 2 months (FDA implant) or daily (off-label injection)
Lowest starting point. Hold about a week to assess tolerance before stepping up.
16 mg implant (FDA) or 0.5-1 mg daily (off-label)
Every 2 months (FDA implant) or daily (off-label injection)
Standard
Photoprotective Tanning
1-2 mg · SubQ · Daily
human clinical trial · FDA Scenesse label
1-2 mg
Daily
Reconstitution CalculatorU-100
050100u
Draw to
units
01

How it works

MT-I binds the melanocortin-1 receptor (MC1R) on melanocytes in skin and hair follicles, activating the cAMP/PKA cascade. PKA phosphorylates the microphthalmia-associated transcription factor (MITF), which upregulates expression of melanogenesis enzymes (tyrosinase, TRP-1, TRP-2). Net effect: increased eumelanin (the photoprotective black/brown melanin) production and pigment transfer to keratinocytes.

MC1R Selectivity

MT-I has 10-100x higher affinity for MC1R than for the other melanocortin receptors (MC3R, MC4R, MC5R). This selectivity contrasts with MT-II's broader profile and explains MT-I's restricted side-effect profile (no significant appetite, libido, or erection effects).

Eumelanin Shift

Activation of MC1R favors eumelanin synthesis over pheomelanin - eumelanin is the photoprotective pigment that absorbs UV radiation. The mechanism is therapeutically meaningful for EPP patients, who lack effective photoprotection due to porphyrin accumulation.

Linear Peptide Stability

Linear 13-amino-acid analog with [Nle4, D-Phe7] substitutions (replacing native Met4 with norleucine and Phe7 with D-phenylalanine). These substitutions resist enzymatic degradation and extend the molecule's bioavailability vs native α-MSH.

Clinical Delivery

FDA-approved as Scenesse implant - 16 mg sustained-release implant placed subcutaneously every 2 months. Provides sustained α-MSH receptor activation over weeks. Off-label use is via daily SubQ injection of reconstituted lyophilized peptide.

No Met or Cys

Linear peptide without methionine or cysteine residues - chemistry-compatible with copper-conjugated peptides like GHK-Cu. No disulfide bonds means no copper-driven oxidation concerns.

02

What to expect

Early
Days 1–7

Week 1-2: existing pigmented spots darken; early tan develops with UV exposure.

Mid
Weeks 2–4

Week 2-4: deeper, more even tan develops with continued UV exposure.

Later
Weeks 4–12

Week 4+: tan stabilizes; maintenance dosing 1-2x weekly sustains. Without UV exposure, MT-I produces little visible effect.

03

Evidence

HumanStrong
AnimalPresent
In-vitroPresent
2015Afamelanotide for erythropoietic protoporphyria · Langendonk JG et al, New England Journal of Medicine ↗peer reviewed
2020Long-term effect and safety of afamelanotide implants in patients with erythropoietic protoporphyria · Wensink D et al, JAMA Dermatology ↗peer reviewed
2010α-MSH-related peptides: a new class of anti-inflammatory and immunomodulating drugs · Catania A et al, Annals of the New York Academy of Sciences ↗review
2016Afamelanotide: a review in erythropoietic protoporphyria · Lyseng-Williamson KA, American Journal of Clinical Dermatology ↗review
04

Interactions & stacking

What's safe to combine, and what belongs in a separate pin.

Keep separate

05

Side effects & safety

Commonly reported

  • Mild injection site reaction
  • Mild nausea (less than MT-II)
  • Mild headache (early doses)
  • Darkening of existing moles and freckles (expected pharmacologic effect)
  • Increased number of visible nevi (some users report new dark spots)

Less common & notes

  • Persistent skin pigmentation in unexpected sites (lips, nipples, genitals) - typically reversible.
  • Theoretical risk of dysplastic nevi activation or melanoma promotion - relative contraindication in patients with melanoma history or significant atypical nevi.
  • Severe injection site reactions are rare.
  • Long-term safety in off-label use beyond Scenesse trial / surveillance data is incompletely characterized.
  • EPP-population safety established through Scenesse Phase 3 trials and post-marketing surveillance.
06

Pharmacokinetics

Illustrative plasma concentration · 24 h
Half-life
Native peptide ~24-48 hours functional (Scenesse implant provides sustained release over weeks)
Peak · Tmax
12-24 hours post-injection (SubQ)
Elimination
Days to weeks (sustained melanin production beyond plasma clearance)
Activity
Days to weeks - melanin production cascade continues after peptide clears

Storage. Lyophilized vial: refrigerate (2-8°C / 36-46°F) or freeze (-20°C) for long-term storage. After reconstitution with bacteriostatic water: refrigerate, use within 21-28 days. LIGHT-SENSITIVE - protect from light especially after reconstitution. Discard if cloudy, discolored, or contains particulates. Scenesse implant: clinician-stored per manufacturer specifications.

07

Regulatory status

FDA-approved as Scenesse (afamelanotide, 16 mg implant) since October 2019 for adult patients with erythropoietic protoporphyria (EPP). EMA-approved 2014 for same indication. Outside EPP, off-label tanning use is unapproved. Sale of MT-I as a research peptide for off-label tanning use is technically prohibited by FDA but enforcement is variable. Some sport organizations prohibit melanocortin agonists under performance-enhancement rules. Pregnancy category data inadequate.

Put it to work

Calculate, log & track

Open MT-I (Melanotan I) straight into OnePin with your vial and dose pre-filled, then let it handle the syringe math and remind you before the vial runs out.

Open MT-I (Melanotan I) in the app →

Go deeper

The guide & community

The complete MT-I (Melanotan I) walkthrough, real protocol discussion and coaching live inside the BlessUp community on Skool.

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