library.onepin.app/matrixyl Peptide Education Library
Education only, not medical advice 21+ Every dose is an example to finalize with a licensed provider

OnePin Peptide Library · Skin

Skin Palmitoyl Pentapeptide-4 (Pal-KTTKS)

Matrixyl

Palmitoyl Pentapeptide-4 · Matrixyl 3000 · Pal-KTTKS

Palmitoyl Pentapeptide-4 (Pal-KTTKS)Lipidated Cosmetic PeptideCollagen-Stimulating CosmeceuticalCosmetic Ingredient (not pharmaceutical)

Matrixyl (Palmitoyl Pentapeptide-4, Pal-KTTKS) is a cosmetic peptide developed by Sederma (Croda) consisting of the 5-amino-acid sequence Lys-Thr-Thr-Lys-Ser conjugated to a palmitic acid (C16) tail. The pentapeptide sequence is derived from the C-terminal collagen propeptide that is normally cleaved during collagen maturation - in skin biology this fragment provides feedback signaling to fibroblasts to make more collagen. The palmitoylation enables transdermal penetration of an otherwise hydrophilic peptide.

Quick Start
Route
Topical (only)
Start low
3-5% formulation
Frequency
1-2x daily
Timing
Independent

Apply to clean skin AM and PM. Combine with moisturizer and SPF (AM). Continuous use required for cumulative effect.

Start here. Every protocol below is ordered lowest-first. Begin at the smallest effective dose, hold about a week to assess tolerance, and step up only if needed.

Tiered Protocols · lowest first
Quick Start
Conservative start
3-5% formulation · Topical (only) · 1-2x daily
Lowest starting point. Hold about a week to assess tolerance before stepping up.
3-5% formulation
1-2x daily
Standard
Collagen Wrinkle Reduction
8-10 % · Topical · Daily
in vitro · Jones et al. 2013, Molecular Pharmaceutics 10(3):1063-1069
8-10 %
Daily
01

How it works

Matrixyl works by mimicking the natural collagen feedback signal:

KTTKS Sequence

The 5-amino-acid peptide sequence Lys-Thr-Thr-Lys-Ser is derived from the C-terminal propeptide of pro-collagen I, which is cleaved during collagen maturation. This cleaved fragment provides paracrine signaling to fibroblasts to upregulate collagen and matrix protein synthesis - completing a feedback loop that maintains tissue repair signaling.

Palmitoylation

The C16 palmitic acid conjugate enables the otherwise hydrophilic pentapeptide to traverse the stratum corneum lipid barrier and reach dermal fibroblasts. Without palmitoylation, KTTKS has minimal topical bioavailability.

Fibroblast Activation

Once internalized by fibroblasts, Matrixyl upregulates expression of collagen type I, collagen type III, fibronectin, glycosaminoglycans, and elastin - the structural matrix proteins that provide skin firmness.

Modest Effect Size

Matrixyl produces measurable but modest improvements in wrinkle depth and skin firmness over 8-12 weeks of consistent topical application. Effect sizes are smaller than retinoids and dramatically smaller than injectable interventions.

No Met or Cys

KTTKS sequence contains no sulfur-containing residues - chemistry-compatible with copper peptides like GHK-Cu in cosmetic formulations.

02

What to expect

Early
Days 1–7

Weeks 1-4: minimal subjective change.

Mid
Weeks 2–4

Weeks 4-12: subtle smoothness and firmness improvement.

Later
Weeks 4–12

Weeks 12+: visible cosmetic effect on fine lines.

03

Evidence

HumanPresent
AnimalPresent
In-vitroPresent
2005Topical palmitoyl pentapeptide provides improved skin function in subjects with photoaged skin · Robinson LR et al, International Journal of Cosmetic Science ↗peer reviewed
2017Cosmeceutical peptides: a review of their efficacy · Schagen SK, Cosmetics (MDPI) ↗review
2020Topical peptides as cosmeceuticals · Errante F et al, Pharmaceutics ↗review
1993Pal-KTTKS, a synthetic peptide containing the natural matrikin sequence, induces collagen and fibronectin synthesis · Katayama K et al (Sederma), Cosmetics & Toiletries ↗industry
04

Interactions & stacking

What's safe to combine, and what belongs in a separate pin.

Keep separate

05

Side effects & safety

Commonly reported

  • Mild skin irritation (rare)
  • Mild redness at application site

Less common & notes

  • Allergic reaction to peptide or formulation.
  • Long-term safety profile is excellent (decades of cosmetic use).
  • Modest effect size means dissatisfaction with magnitude is the most common 'complaint.'
06

Pharmacokinetics

Illustrative plasma concentration · 24 h
Half-life
Topical; minimal systemic absorption
Peak · Tmax
Topical 2-4 hours
Elimination
Days to weeks for cumulative effect
Activity
Continuous use for sustained effect

Storage. Topical formulations: room temperature stable per manufacturer. Lyophilized peptide: refrigerate, light-sensitive.

07

Regulatory status

Cosmetic ingredient (not pharmaceutical). Approved as cosmetic ingredient in EU, US, and most jurisdictions. Sederma/Croda maintains the ingredient registration.

Put it to work

Calculate, log & track

Open Matrixyl straight into OnePin with your vial and dose pre-filled, then let it handle the syringe math and remind you before the vial runs out.

Open Matrixyl in the app →

Go deeper

The guide & community

The complete Matrixyl walkthrough, real protocol discussion and coaching live inside the BlessUp community on Skool.

Join the community ↗
Open in app Community