library.onepin.app/kisspeptin Peptide Education Library
Education only, not medical advice 21+ Every dose is an example to finalize with a licensed provider

OnePin Peptide Library · Hormonal

Hormonal Endogenous neuropeptide Not FDA-approved · investigational

Kisspeptin

Kisspeptin-10 · KP-10 · Metastin

Endogenous neuropeptideGnRH neuron activatorHPG axis upstream regulatorReproductive hormone modulator

Kisspeptin is a peptide encoded by the KISS1 gene that acts as the master upstream regulator of the reproductive hormone axis. It stimulates GnRH (gonadotropin-releasing hormone) neurons in the hypothalamus, which in turn triggers LH and FSH release from the pituitary, driving testosterone production and spermatogenesis. Kisspeptin acts upstream of GnRH, making it the most physiologically natural way to stimulate the entire HPG (hypothalamic-pituitary-gonadal) axis.

Quick Start
Route
SubQ injection
Start low
Protocol-dependent
Frequency
As-needed or scheduled protocol
Timing
No fasting required

Per physician direction. Timing depends on clinical indication.

Start here. Every protocol below is ordered lowest-first. Begin at the smallest effective dose, hold about a week to assess tolerance, and step up only if needed.

Tiered Protocols · lowest first
Quick Start
Conservative start
Protocol-dependent · SubQ injection · As-needed or scheduled protocol
Lowest starting point. Hold about a week to assess tolerance before stepping up.
Protocol-dependent
As-needed or scheduled protocol
Intermediate
LH Surge / TRT Adjunct
10-20 mcg · SubQ · Daily
human study · Abbara, Jayasena et al. 2018 (Neuroendocrinology)
10-20 mcg
Daily
Reconstitution CalculatorU-100
050100u
Draw to
units
01

How it works

Kisspeptin binds to the KISS1R (GPR54) receptor on GnRH neurons in the hypothalamus, triggering GnRH pulsatile release. This cascade stimulates pituitary LH and FSH secretion, which drives testicular testosterone production and spermatogenesis. Unlike HCG (which bypasses the hypothalamus/pituitary by directly stimulating Leydig cells), Kisspeptin preserves the entire HPG feedback loop.

Kisspeptin binds to the KISS1R (GPR54) receptor on GnRH neurons in the hypothalamus, triggering GnRH pulsatile release. This cascade stimulates pituitary LH and FSH secretion, which drives testicular testosterone production and spermatogenesis. Unlike HCG (which bypasses the hypothalamus/pituitary by directly stimulating Leydig cells), Kisspeptin preserves the entire HPG feedback loop.

02

What to expect

Early
Days 1–7

Hours: LH elevation detectable.

Mid
Weeks 2–4

Days-weeks: Testosterone response.

Later
Weeks 4–12

Ongoing: HPG axis modulation.

03

Evidence

HumanPresent
AnimalStrong
In-vitroPresent
2025Kisspeptin Administration Stimulates Reproductive Hormones but Does Not Affect Anxiety in Humans · Mills EGA et al, Journal of Clinical Endocrinology & Metabolism ↗peer reviewed
2011The Effects of Kisspeptin-10 on Reproductive Hormone Release Show Sexual Dimorphism in Humans · Chan YM et al, Journal of Clinical Endocrinology & Metabolism ↗peer reviewed
2020Kisspeptin receptor agonist has therapeutic potential for female reproductive disorders · Abbara A et al, Journal of Clinical Investigation ↗peer reviewed
2021Clinical Potential of Kisspeptin in Reproductive Health · Mills EG et al, Trends in Molecular Medicine ↗review
04

The honest take

Most physiological HPG stimulation

Confirmed. Kisspeptin is the most upstream activator of the reproductive axis, preserving all natural feedback mechanisms.

05

Interactions & stacking

What's safe to combine, and what belongs in a separate pin.

Safe & synergistic

HCGKisspeptin stimulates GnRH upstream, HCG stimulates Leydig cells downstream. Different HPG levels.

Keep separate

ALL (syringe)PIN ALONE.
06

Side effects & safety

Commonly reported

  • Injection site reaction
  • Warmth or flushing

Less common & notes

  • Limited adverse event data.
  • Endogenous peptide suggesting good tolerability.
07

Pharmacokinetics

Illustrative plasma concentration · 24 h
Half-life
0.47h
Peak · Tmax
0.25h
Elimination
2.35h
Activity
2-6 hours (LH/testosterone elevation)

Storage. Standard BAC water 2-8C. 28 days.

08

Regulatory status

Not FDA-approved for routine use. Used in IVF research. Available as research peptide.

Put it to work

Calculate, log & track

Open Kisspeptin straight into OnePin with your vial and dose pre-filled, then let it handle the syringe math and remind you before the vial runs out.

Open Kisspeptin in the app →

Go deeper

The guide & community

The complete Kisspeptin walkthrough, real protocol discussion and coaching live inside the BlessUp community on Skool.

Join the community ↗
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