Hormonal Endogenous neuropeptideNot FDA-approved · research compound

Kisspeptin

Kisspeptin-10 · KP-10 · Metastin

Evidence: Studied in humans. Example dose: finalize with a provider. Not a fact-check stamp.

Protocol-dependent – 10-20 mcg SubQ As-needed or scheduled protocol

GnRH neuron activatorHPG axis upstream regulatorReproductive hormone modulator

Kisspeptin is a peptide encoded by the KISS1 gene that acts as the master upstream regulator of the reproductive hormone axis. It stimulates GnRH (gonadotropin-releasing hormone) neurons in the hypothalamus, which in turn triggers LH and FSH release from the pituitary, driving testosterone production and spermatogenesis. Kisspeptin acts upstream of GnRH, making it the most physiologically natural way to stimulate the entire HPG (hypothalamic-pituitary-gonadal) axis.

Quick Start

Route

SubQ injection

Start low

Protocol-dependent SubQ

Frequency

As-needed or scheduled protocol

Timing

No fasting required

Starting dose. Protocol-dependent

Per physician direction. Timing depends on clinical indication.

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Start here. Every protocol below is ordered lowest-first. Begin at the smallest effective dose, hold about a week to assess tolerance, and step up only if needed.

Tiered Protocols · lowest first

Conservative start

Protocol-dependent · SubQ injection · As-needed or scheduled protocol

conservative starter

Protocol-dependent

As-needed or scheduled protocol

Intermediate

LH Surge / TRT Adjunct

10-20 mcg · SubQ · Daily

human study · Abbara, Jayasena et al. 2018 (Neuroendocrinology)

Extremely short half-life requires daily or multiple daily injections. Does not directly shut down natural HPTA like exogenous hormones. Note the route: the cited study administered kisspeptin-54 INTRAVENOUSLY. This card is subcutaneous, which was not the studied route.

10-20 mcg

Daily

Reconstitution CalculatorU-100

Vial strength

BAC water

Vials in this mix

Syringe size

Target dose

050100u

Draw to

— units

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I will split this dose across more than one syringe

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How it works

Kisspeptin binds to the KISS1R (GPR54) receptor on GnRH neurons in the hypothalamus, triggering GnRH pulsatile release. This cascade stimulates pituitary LH and FSH secretion, which drives testicular testosterone production and spermatogenesis. Unlike HCG (which bypasses the hypothalamus/pituitary by directly stimulating Leydig cells), Kisspeptin preserves the entire HPG feedback loop.

What to expect

Early

Days 1–7

Hours: LH elevation detectable.

Mid

Weeks 2–4

Days-weeks: Testosterone response.

Later

Weeks 4–12

Ongoing: HPG axis modulation.

Evidence

HumanPresent

AnimalStrong

In-vitroPresent

Each reference below was re-fetched from its PubMed record, and the quoted sentence was confirmed to appear in that abstract and to support the claim it sits under. Follow any of them to read the source.

Evidence boundary from the checked abstract: Recent studies have shown that kisspeptin neurons located in arcuate nucleus (ARC) co-express neurokinin B (NKB) and dynorphin (Dyn).

Recent studies have shown that kisspeptin neurons located in arcuate nucleus (ARC) co-express neurokinin B (NKB) and dynorphin (Dyn).

The Role of Kisspeptin in the Control of the Hypothalamic-Pituitary-Gonadal Axis and Reproduction. Frontiers in endocrinology · 2022 · PMID 35837314

Evidence scope. This entry supports only the exact statement quoted from the live abstract; it does not independently verify OnePin's displayed dose, route, frequency, formulation, population, or broader mechanism.

Claims we could not support

No abstract we checked supports these for this molecule at this route. That is not the same as saying they are false — it marks where the evidence is missing.

Dose/route: Protocol-dependent; SubQ injection; As-needed or scheduled protocol. No checked live PubMed abstract verified this exact displayed dose, route, and frequency for the same molecule/formulation and relevant population.

Primary safety claim: No primary safety text present in source JSON. No checked live PubMed abstract directly verified this source-JSON safety claim at the displayed formulation, route, and regimen.

The honest take

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Most physiological HPG stimulation

Confirmed. Kisspeptin is the most upstream activator of the reproductive axis, preserving all natural feedback mechanisms.

Interactions & stacking

Two different questions: what is documented about running these together, and what is documented about drawing them into the same syringe. A good pharmacological partner can still have to be pinned separately.

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Pin alone. Kisspeptin must not share a syringe with anything else, whatever the pharmacology says. PIN ALONE.

Documented together

HCGKisspeptin stimulates GnRH upstream, HCG stimulates Leydig cells downstream. Different HPG levels.

Check any specific pair in the Stack & Interaction Checker, which answers both questions separately. Absence of a documented conflict is not evidence of safety.

Side effects & safety

Commonly reported

Injection site reaction
Warmth or flushing

Less common & notes

Limited adverse event data.
Endogenous peptide suggesting good tolerability.

Pharmacokinetics

Illustrative plasma concentration · 2.1 h

Half-life

0.47h

Peak · Tmax

0.25h

Elimination

2.35h

Bioavailability

~100%

SubQ

Duration of action

2-6 hours

LH/testosterone elevation

Clearance

Enzymatic degradation (matrix metalloproteinases and furin)

Storage. Standard BAC water 2-8C. 28 days.

Regulatory status

Not FDA-approved for routine use. Used in IVF research. Available as research peptide.

Put it to work

Calculate, log & track

Open Kisspeptin straight into OnePin with your vial and dose pre-filled, then let it handle the syringe math and remind you before the vial runs out.

Open Kisspeptin in the app →

Go deeper

The guide & community

The complete Kisspeptin walkthrough, real protocol discussion and coaching live inside the BlessUp community on Skool.

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