library.onepin.app/bromelain Peptide Education Library

Education only, not medical advice◆ 21+◆ Every dose is an example to finalize with a licensed provider

OnePin Peptide Library · Gut & Digestion

Gut & Digestion Pineapple Proteolytic Enzyme

Bromelain

Pineapple Enzyme

Pineapple Proteolytic EnzymeCysteine Protease ComplexAnti-Inflammatory + Digestive EnzymeDietary Supplement

Bromelain is a proteolytic enzyme complex extracted from pineapple stem and fruit. Composed of multiple cysteine proteases, it has both digestive and systemic anti-inflammatory effects when taken orally on an empty stomach (digestive use is with-meal; systemic use is between meals).

Quick Start

Route

Oral capsule

Start low

500mg (1,200-2,400 GDU)

Frequency

2-3x daily

Timing

Empty stomach (anti-inflammatory) OR with meals (digestion)

Empty stomach for systemic effects.

❋

Start here. Every protocol below is ordered lowest-first. Begin at the smallest effective dose, hold about a week to assess tolerance, and step up only if needed.

Tiered Protocols · lowest first

Quick Start

Conservative start

500mg (1,200-2,400 GDU) · Oral capsule · 2-3x daily

Lowest starting point. Hold about a week to assess tolerance before stepping up.

500mg (1,200-2,400 GDU)

2-3x daily

Standard

Anti-Inflammatory / Proteolytic

500-1000 mg · Oral · 2x Daily

human study · Brien et al. 2004 (Evid Based Complement Alternat Med; PMID 15841258)

500-1000 mg

2x Daily

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How it works

Bromelain has dual mechanisms based on timing:

Empty Stomach (Anti-Inflammatory)

Absorbed intact (small fraction) into bloodstream where it modulates inflammation - reduces bradykinin, fibrin, and pro-inflammatory cytokines. Mechanism includes plasmin activation and complement modulation.

With Meals (Digestive)

Hydrolyzes dietary proteins in the stomach and small intestine - improves protein digestion in users with low pancreatic enzyme output.

Anti-Edema

Reduces post-surgical and traumatic swelling - well-documented in dental/orthopedic surgery contexts.

Mucolytic

Thins mucus in sinusitis.

What to expect

Early

Days 1–7

Days 1-7: post-surgical edema reduction.

Mid

Weeks 2–4

Weeks 1-4: peak anti-inflammatory effects.

Later

Weeks 4–12

Long-term: as needed.

Evidence

HumanPresent

AnimalStrong

In-vitroPresent

2012Bromelain: an overview · Pavan R et al, Biotechnology Research International ↗review

2001The use of bromelain in inflammation · Maurer HR, Cellular and Molecular Life Sciences ↗review

2016Effects of bromelain on post-surgical edema · Rathnavelu V et al, Biomedical Reports ↗peer reviewed

Interactions & stacking

What's safe to combine, and what belongs in a separate pin.

Keep separate

Side effects & safety

Commonly reported

Mild GI upset
Mild allergic skin reactions

Less common & notes

Bleeding risk with anticoagulants.
Pineapple cross-reactivity.
Hypotension at high doses (rare).

Pharmacokinetics

Illustrative plasma concentration · 24 h

Half-life

Hours for absorbed fraction

Peak · Tmax

1-3 hours post-ingestion

Elimination

Hours plasma; cumulative effects over weeks

Activity

Daily dosing for sustained effects

Storage. Capsule. Cool dry location, refrigerate for extended storage.

Regulatory status

Dietary supplement (US, DSHEA). Pharmaceutical use in some EU countries.

Put it to work

Calculate, log & track

Open Bromelain straight into OnePin with your vial and dose pre-filled, then let it handle the syringe math and remind you before the vial runs out.

Open Bromelain in the app →

Go deeper

The guide & community

The complete Bromelain walkthrough, real protocol discussion and coaching live inside the BlessUp community on Skool.

Join the community ↗

Open in app Community