library.onepin.app/hgh-frag-176-191 Peptide Education Library
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OnePin Peptide Library · Weight

Weight HGH C-Terminal Fragment (residues 176-191) Not FDA-approved · investigational

HGH Frag 176-191

HGH Fragment 176-191 · Lipolytic Fragment · Frag 176-191

HGH C-Terminal Fragment (residues 176-191)Lipolytic PeptideGH Activity-Selective (lipolysis without anabolic action)Research Chemical (no human approval)

HGH Frag 176-191 is the native 16-amino-acid C-terminal fragment of human growth hormone (residues 176-191), corresponding to a beta-turn region of the parent molecule that contains the lipolytic activity of GH without the broader anabolic, glycemic, or growth-promoting effects of full-length GH. The native frag is a research-only compound and is the precursor / parent structure to AOD-9604 (Anti-Obesity Drug 9604), which is a stabilized version with an added N-terminal tyrosine for enhanced stability and potency.

Quick Start
Route
Subcutaneous (SubQ) injection
Start low
200-300mcg
Frequency
1-2x daily
Timing
Fasted preferred for lipolysis effect; pre-cardio common

Inject fasted morning and/or pre-workout. Reconstitute with bacteriostatic water; refrigerate. 30-60 minute half-life means timing relative to fasted-state cardio matters. The native fragment is less stable than AOD-9604 - use within 14-21 days post-reconstitution.

Start here. Every protocol below is ordered lowest-first. Begin at the smallest effective dose, hold about a week to assess tolerance, and step up only if needed.

Tiered Protocols · lowest first
Quick Start
Conservative start
200-300mcg · Subcutaneous (SubQ) injection · 1-2x daily
Lowest starting point. Hold about a week to assess tolerance before stepping up.
200-300mcg
1-2x daily
Standard
Fasted Lipolysis
250-500 mcg · SubQ · Daily
animal study · Ng et al. 2000 (Hormone Research 53(6):274-278)
250-500 mcg
Daily
Reconstitution CalculatorU-100
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01

How it works

HGH Frag 176-191 binds the beta-3 adrenergic receptor (β3-AR) on adipocytes and activates the lipolytic cascade similar to other β3 agonists, while also modulating expression of lipid-metabolism genes via mechanisms not fully characterized.

β3-Adrenergic Receptor Activation

Binds β3-AR on white and brown adipocytes, activating Gs/cAMP/PKA cascade. Hormone-sensitive lipase (HSL) is phosphorylated and translocated to lipid droplets, hydrolyzing triglycerides into free fatty acids and glycerol.

Lipogenesis Suppression

Reduces expression of lipogenic enzymes (acetyl-CoA carboxylase, fatty acid synthase) - net opposes new fat storage even in caloric surplus contexts.

GH Activity Selectivity

Despite originating from GH, the fragment does NOT bind the GH receptor and does NOT raise IGF-1. This eliminates the insulin antagonism, edema, and growth-promoting concerns of intact GH while retaining the lipolytic action.

Met-Containing

The native sequence contains methionine residues. NEVER co-store with copper-conjugated peptides (GHK-Cu) - copper drives oxidation of Met to methionine sulfoxide, degrading the peptide.

Stability vs AOD-9604

The native fragment is less stable than AOD-9604 (which has an added N-terminal tyrosine). Native frag should be reconstituted only when needed and stored cold; AOD-9604 has better shelf stability.

Short Plasma Half-Life

~30-60 minutes plasma; functional lipolytic effect 2-4 hours per dose. Daily dosing typical, often split AM and pre-workout.

02

What to expect

Early
Days 1–7

Week 1-2: minimal subjective effect. Lipolytic effect is silent at the cellular level.

Mid
Weeks 2–4

Week 4-6: in caloric deficit, may marginally accelerate fat loss vs diet alone.

Later
Weeks 4–12

Week 8-12: cumulative body composition reflects diet + cardio + drug. Standalone effect likely modest based on AOD-9604 clinical data.

03

Evidence

HumanModerate
AnimalStrong
In-vitroPresent
1978The lipolytic effects of fragments of human growth hormone · Ng FM, Bornstein J, Diabetes ↗peer reviewed
2000Effects of human growth hormone-(177-191) on body weight, blood glucose and food intake in genetically obese mice · Heffernan MA et al, Endocrine Research ↗peer reviewed
2001The effects of human GH and its lipolytic fragment (AOD9604) on lipid metabolism following chronic treatment in obese mice and beta3-AR knock-out mice · Heffernan M et al, Endocrinology ↗peer reviewed
2001AOD9604 - a fragment derived from human growth hormone - has lipolytic effects without GH-like effects · Heffernan M et al, Diabetes Obesity & Metabolism ↗peer reviewed
04

Interactions & stacking

What's safe to combine, and what belongs in a separate pin.

Keep separate

05

Side effects & safety

Commonly reported

  • Injection site reaction (mild)
  • Mild headache (early doses)
  • Possible mild tachycardia (β3-AR mediated, typically mild)

Less common & notes

  • Mild gastrointestinal upset.
  • Theoretical β3-AR-mediated cardiovascular effects (mild tachycardia, palpitations) - typically mild.
  • Long-term safety beyond Phase 2b AOD-9604 data is uncharacterized for native HGH frag.
  • Methionine oxidation reduces potency over storage time - users dose escalating may be compensating for degraded peptide rather than gaining efficacy.
  • Allergic reaction to peptide is rare.
06

Pharmacokinetics

Illustrative plasma concentration · 24 h
Half-life
~30-60 minutes plasma
Peak · Tmax
~30 minutes (SubQ)
Elimination
2-4 hours functional lipolytic window
Activity
Daily or split-daily dosing typical

Storage. Lyophilized vial: refrigerate (2-8°C / 36-46°F) or freeze (-20°C) for long-term storage. After reconstitution with bacteriostatic water: refrigerate, use within 14-21 days. The native fragment is less stable than AOD-9604 - methionine residues are oxidation-sensitive. Light-sensitive. Do NOT shake. Discard if cloudy, discolored, or beyond use-by window.

07

Regulatory status

Not FDA-approved for any indication. Native HGH Frag 176-191 never advanced to clinical trials. The stabilized derivative AOD-9604 advanced through Phase 2b trials in obesity (Metabolic Pharmaceuticals) but failed primary endpoint and was abandoned. Sold only as research chemical from peptide-supply vendors. WADA prohibited under class S2 (peptide hormones, growth factors). Sale for human use is illegal under FDA regulation.

Put it to work

Calculate, log & track

Open HGH Frag 176-191 straight into OnePin with your vial and dose pre-filled, then let it handle the syringe math and remind you before the vial runs out.

Open HGH Frag 176-191 in the app →

Go deeper

The guide & community

The complete HGH Frag 176-191 walkthrough, real protocol discussion and coaching live inside the BlessUp community on Skool.

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