library.onepin.app/retatrutide Peptide Education Library
Education only, not medical advice 21+ Every dose is an example to finalize with a licensed provider

OnePin Peptide Library · Metabolic

Metabolic Triple hormone receptor agonist (GLP-1/GIP/glucagon) Not FDA-approved · investigational

Retatrutide

Reta · Tri-G · GGG

Triple hormone receptor agonist (GLP-1/GIP/glucagon)Incretin-based peptideAnti-obesity investigational drugLong-acting injectable peptide

Retatrutide (LY3437943) is a first-in-class triple agonist peptide targeting GLP-1 (glucagon-like peptide-1), GIP (glucose-dependent insulinotropic polypeptide), and glucagon receptors simultaneously. Developed by Eli Lilly, it produced the most dramatic weight loss results of any obesity peptide in Phase 2 trials, with participants losing up to 24.2% of body weight at 48 weeks at the highest dose. The triple agonist mechanism addresses appetite suppression (GLP-1), insulin sensitivity and fat metabolism (GIP), and energy expenditure and hepatic fat reduction (glucagon). Retatrutide represents the next generation beyond dual agonists like tirzepatide.

Quick Start
Route
SubQ injection
Start low
Start low (1-2 mg weekly) and titrate up slowly
Frequency
Weekly or twice weekly (compounded versions)
Timing
No strict fasting requirement. Take with or without food.

Any time of day, with or without food. Once weekly.

Start here. Every protocol below is ordered lowest-first. Begin at the smallest effective dose, hold about a week to assess tolerance, and step up only if needed.

Tiered Protocols · lowest first
Quick Start
Conservative start
Start low (1-2 mg weekly) and titrate up slowly · SubQ injection · Weekly or twice weekly (compounded versions)
Lowest starting point. Hold about a week to assess tolerance before stepping up.
Start low (1-2 mg weekly) and titrate up slowly
Weekly or twice weekly (compounded versions)
Expert
Triple-Agonist Titration
1 mg · SubQ · Weekly
human clinical trial · Jastreboff et al. 2023 (N Engl J Med)
1 mg
Weekly
Reconstitution CalculatorU-100
050100u
Draw to
units
01

How it works

Retatrutide activates three receptor systems simultaneously. GLP-1 receptor activation suppresses appetite via hypothalamic signaling, slows gastric emptying, and enhances glucose-dependent insulin secretion. GIP receptor activation improves insulin sensitivity, enhances lipid metabolism in adipose tissue, and may reduce inflammation. Glucagon receptor activation increases hepatic energy expenditure, promotes lipolysis, reduces hepatic fat accumulation, and increases thermogenesis. The glucagon component is what distinguishes retatrutide from tirzepatide (GLP-1/GIP only) and semaglutide (GLP-1 only), and is believed to account for the superior weight loss results through increased energy expenditure.

Retatrutide activates three receptor systems simultaneously. GLP-1 receptor activation suppresses appetite via hypothalamic signaling, slows gastric emptying, and enhances glucose-dependent insulin secretion. GIP receptor activation improves insulin sensitivity, enhances lipid metabolism in adipose tissue, and may reduce inflammation. Glucagon receptor activation increases hepatic energy expenditure, promotes lipolysis, reduces hepatic fat accumulation, and increases thermogenesis. The glucagon component is what distinguishes retatrutide from tirzepatide (GLP-1/GIP only) and semaglutide (GLP-1 only), and is believed to account for the superior weight loss results through increased energy expenditure.

02

What to expect

Early
Days 1–7

Weeks 1-4: Appetite suppression noticeable. Nausea common during titration. Initial weight loss primarily from reduced caloric intake. Slow titration minimizes side effects.

Mid
Weeks 2–4

Weeks 4-12: Consistent weight loss. GI side effects typically improve. Body composition changes visible. Blood glucose improvements if metabolically impaired.

Later
Weeks 4–12

Weeks 12-48: Significant cumulative weight loss. Phase 2 showed 17-24% body weight loss depending on dose. Hepatic fat reduction. Metabolic markers improve substantially.

03

Evidence

HumanPresent
AnimalPresent
In-vitroPresent
2023Triple-Hormone-Receptor Agonist Retatrutide for Obesity - A Phase 2 Trial · Jastreboff AM, Kaplan LM, Frias JP et al, New England Journal of Medicine ↗peer reviewed
2023Retatrutide, a GIP, GLP-1 and glucagon receptor agonist, for people with type 2 diabetes: a randomised, double-blind, placebo and active-controlled, parallel-group, phase 2 trial conducted in the USA · Rosenstock J, Frias J, Jastreboff AM et al, The Lancet ↗peer reviewed
2024Triple hormone receptor agonist retatrutide for metabolic dysfunction-associated steatotic liver disease: a randomized phase 2a trial · Sanyal AJ, Kaplan LM, Frias JP et al, Nature Medicine ↗peer reviewed
04

The honest take

Most potent weight loss peptide

Supported

Confirmed by Phase 2 data. 24.2% body weight loss exceeds semaglutide (15-16%) and tirzepatide (20-22%). Triple agonist mechanism provides additive weight loss.

Hepatic fat reduction

Glucagon receptor activation specifically targets hepatic fat. Phase 2 data showed significant liver fat reduction. Promising for NASH/NAFLD.

Energy expenditure increase

Glucagon component increases basal metabolic rate. This distinguishes retatrutide from GLP-1-only agents that primarily reduce intake.

05

Interactions & stacking

What's safe to combine, and what belongs in a separate pin.

Safe & synergistic

BPC-157BPC-157 supports GI mucosal healing, may reduce GLP-1 related nausea and gastroparesis. Pin separately.
AOD-9604Retatrutide suppresses appetite, AOD targets fat metabolism directly. Complementary weight management. Pin separately.

Keep separate

ALL (syringe)PIN ALONE. GLP-1 class. FDA labeling for all GLP-1 agonists specifies separate injections.
SemaglutideDo not stack GLP-1 agonists. Overlapping receptor activation increases nausea, GI distress, and hypoglycemia risk.
TirzepatideDo not stack GLP-1 agonists.
MetforminBoth lower blood glucose. Monitor for hypoglycemia. Dose adjustment may be needed.
06

Side effects & safety

Commonly reported

  • Nausea (most common, dose-dependent)
  • Diarrhea
  • Decreased appetite
  • Vomiting during titration
  • Constipation

Less common & notes

  • Gastroparesis (delayed gastric emptying) with chronic use.
  • Pancreatitis risk (class effect).
  • Gallbladder issues with rapid weight loss.
  • Heart rate increase.
  • Monitor thyroid (GLP-1 class has thyroid C-cell tumor signal in rodents).
  • Contraindicated with personal/family history of medullary thyroid carcinoma or MEN2.
  • Oral contraceptives may be less reliable during dose initiation and escalation: delayed gastric emptying (GLP-1 / amylin) can reduce oral drug absorption - Tirzepatide carries an FDA label warning to use a backup or non-oral method for 4 weeks after starting and after each dose increase.
07

Pharmacokinetics

Illustrative plasma concentration · 24 h
Half-life
144h
Peak · Tmax
24h
Elimination
720h
Activity
7 days (supports weekly dosing; appetite suppression sustained between doses)

Storage. Refrigerate at 2-8C. Protect from light. Stable for 28 days after reconstitution. GLP-1 class peptides are generally stable. Do not freeze after reconstitution.

08

Regulatory status

Investigational drug (Eli Lilly). Phase 3 trials ongoing. Not yet FDA-approved. Available through compounding pharmacies. WADA status: Not specifically listed but metabolic modulators may fall under S4.

Put it to work

Calculate, log & track

Open Retatrutide straight into OnePin with your vial and dose pre-filled, then let it handle the syringe math and remind you before the vial runs out.

Open Retatrutide in the app →

Go deeper

The guide & community

The complete Retatrutide walkthrough, real protocol discussion and coaching live inside the BlessUp community on Skool.

Join the community ↗
Open in app Community